MicroRNA protocols /

Ying, Shao-Yao

MicroRNA protocols / edited by Shao-Yao Ying. - Totowa, N.J. : Humana Press, c2006. - xiii, 365 p. : ill. (some col.) ; 24 cm. - Methods in molecular biology, 342 1064-3745 ; . - Methods in molecular biology (Clifton, N.J.) ; v. 342. .

Includes bibliographical references and index.

The microRNA: overview of the RNA gene that modulates gene functions
Structure analysis of microRNA precursors
MicroRNA biogenesis: isolation and characterization of the microprocessor complex Recognition and cleavange of primary microRNA transcripts
Mouse embryonic stem cells as a model genetic system to dissect and exploit the RNA interference machinery MicroRNAs and messenger RNA turnover
Prediction of microRNA targets
Prediction of human microRNA targets
Complications in mammalian microRNA target prediction miRBase: the microRNA sequence database In situ hybridization as a tool to study the role of microRNAs in plant development
Usefulness of the luciferase reporter system to test in efficacy of siRNA Cloning microRNAs from mammalian tissues Methods for analyzing microRNA expression and function during hematopoietic lineage differentiation Identifying microRNNA regulators of cell death in Drosophila
MicroRNAs in human immunodeficiency virus-1 infection Cloning and detection of HIV-1-encoded microRNA microRNAs associated with fragile X mental retardation protein In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector Gene silencing in vitro and in vivo using intronic microRNAs Transgene-link animal models using intronic microRNAs
Evolution of microRNAs
Perspectives


It is now known that microRNA (miRNA), once thought to be junk, can suppress the expression of other genes and may be involved in numerous cellular processes, including cell proliferation, apoptosis, cell differentiation, and development. In MicroRNA Protocols, expert miRNA researchers explain its basic concepts and introduce the most advanced technologies and techniques for predicting screening, isolating, and Read more...

1588295818 (alk. paper) 1597451231 (eISBN) 9781588295811


Small interfering RNA.
MicroRNA.
MicroRNAs.

572.88 / YAO

W1 / ME9616J v.342 2006 QU 58.7 / M626 2006

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