Molecular diagnosis of infectious diseases / edited by Jochen Decker, Udo Reischl ; foreword by Christoph Schnorr and Michael Schwörer.

By:

Decker, Jochen

Contributor(s):

Reischl, Udo

Material type: Text

1588292215
9781588292216

Subject(s): Genre/Form:

Laboratory manuals.

Additional physical formats: Print version:: Molecular diagnosis of infectious diseases.DDC classification:

616.9047582 21 DEC

Online resources:

Contents:

PART I. PROTEIN-BASED IDENTIFICATION OF ANTIGENS PART II. DNA-BASED IDENTIFICATION OF ANTIGENS PART III. IDENTIFICATION OF EPITOPES PART IV. EXPRESSION OF RECOMBINANT PROTEINS. PART V. PURIFICATION, MODIFICATION, AND RENATURATION OF RECOMBINANT PROTEINS. PART VI. CHARACTERIZATION OF RECOMBINANT PROTEINS. PART VII. EVALUATION OF RECOMBINANT PROTEINS IN IMMUNOLOGICAL TEST SYSTEMS. PART VIII. RECOMBINANT RECEPTOR MOLECULES

Summary: In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

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Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
General Books	CUTN Central Library Medicine, Technology & Management	Non-fiction	616.9047582 DEC (Browse shelf(Opens below))	Available		41769

In this second edition of a much-acclaimed classic laboratory manual, Molecular Diagnosis of Infectious Diseases, leading experts from academia and industry describe cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors demonstrate the power of these new methodologies in both established and novel procedures by applying them successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, and their use in diagnostic reagents in immunological assays. Basic problems of serological laboratory diagnostics and the use of immunoproteomics for discovering target proteins are also addressed. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding known pitfalls, and extensive references. Comprehensive and up-to-date, Molecular Diagnosis of Infectious Diseases, Second Edition offers novice investigators an easy entry into the field and experienced researchers a broad array of time saving tools that will not only elucidate new principles, but also greatly facilitate the development and successful application of rapid diagnostic methods.

Includes bibliographical references and index.

English.

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