Plant Biotechnology / In Vitro Principles Techniques And Applications: Dr. Mahipal Singh Shekhawat 1, Dr. Vikrant /

1. In vitro plant biotechnology : status and scope. 2. In vitro plant regeneration: an overview. 3. In vitro culture laboratory-organization and management. 4. Sterilization techniques. 5. Plant cell in vitro nutrition: culture medium. 6. Cell differentiation and totipotency. 7. Micropropagation: a source of clonal regeneration. 8. Callus: induction and differentiation. 9. Cell suspension culture. 10. Single cell culture: technology and applications. 11. Embryoculture. 12. Somatic embryo: induction and regeneration. 13. Haploid production-I (Androgenesis). 14. Haploid production II (In vitro pollination fertilization and gynogenesis). 15. Endosperm and nucellus culture. 16. Protoplast technology-isolation and regeneration of protoplast. 17. Protoplast technology-somatic hybridization and cybridization. 18. Somaclonal variation: source and significance. 19. Biodiversity and preservation of germplasm. 20. Artificial (Synthetic) seed production technology. 21. Secondary metabolite production-1. 22. Secondary metabolite production II. 23. Transgenic production I. 24. Transgenic production II. 25. Transgenic production III. 26. GM crops and their impacts. 27. Plastic engineering. 28. Plant in vitro biotechnology in agriculture. 29. Plant in vitro biotechnology in forestry. 30. Plant in vitro biotechnology in Industry. Glossary.

This book explains new and rapidly growing concepts and technologies connected with in vitro plant regeneration and transgenesis and provides an in-depth coverage of all major topics of plant in vitro technology. The vast literature on the subject has been presented in a concise and digestible form thoroughly covering the fundamental as well as applied aspects of the subject This book would be an ideal source of comprehensive, authoritative and up-to-date information for undergraduates, post-graduates and researchers in the field of plant biotechnology. . ;In vitro Plant Biotechnology: Status and ; Scope ; In vitro Plant Regeneration—An Overview In vitro Culture Laboratory— Organization and ; Management ; Sterilization Techniques ; Plant Cell In vitro Nutrition: Culture Medium ; Cell Differentiation and Totipotency Micropropagation: A Source of Clonal ; Regeneration ; Callus: Induction and Differentiation ; Cell Suspension Culture Single Cell Culture: Technology and Applications ; ; Embryo Culture ; Somatic Embryo: Induction and Regeneration ; Haploid Production-I (Androgenesis) Haploid Production-II (In vitro ; Pollination Fertilization and Gynogenesis) ; Endosperm and Nucellus Culture Protoplast Technology— Isolation and ; Regeneration of Protoplast Protoplast Technology— Somatic Hybridization ; and Cybridization ; Somaclonal Variation: Source and Significance ; Biodiversity and Preservation of Germplasm Artificial (synthetic) Seed Production Technology ; ;Secondary Metabolite Production-I ; Secondary Metabolite Production-II ; Transgenic Production-I ; Transgenic Production-II ; Transgenic Production-III ; GM Crops and their Impacts ; Plastid Engineering ; Plant In vitro Biotechnology in Agriculture ; Plant In vitro Biotechnology in Forestry ; Plant In vitro Biotechnology in Industry ; Glossary ; Index Colour Plates CONTENTS Dr. Mahipal Singh Shekhawat, Assistant Professor, KM Centre for PG studies, Pondicherry, obtained his Ph.D. in Botany from Biotechnology Centre, J.N.V. University, Jodhpur, in 2000. He worked as a Post-Doctoral Research Associate in the same Department during 2000–02. Then he joined the Centre for Plant Biotechnology, Hissar (Haryana), as Senior Scientific Officer and worked there for two years (2002–03). He has 10 years of teaching and 13 years of research experience in the field of Plant Biotechnology and has published several research papers in national and international journals. His areas of interest are in vitro production of secondary metabolites and somatic cell genetics of economically important plants. Dr. Vikrant, Assistant Professor, AGCW, Karaikal, Pondicherry (U.T.), obtained his Ph.D. in Botany from the University of Delhi in 2000. During his post-doctoral experience, he worked at Department of Plant Molecular Biology, University of Delhi South Campus and Institute of Biotechnology, University of Texas Health Science Centre, San Antonio (USA), during 2000–03. He has 7 years of teaching and 16 years of research experience in the field of Plant biotechnology and Plant Molecular biology. He has published several research articles in reputed international journals. His area of interest is Stress Molecular Biology of Plants. For Biotechnology, Botany, Microbiology, Biochemistry, Bioinformatics, Agriculture and Environmental Sciences courses, NET, SLET, UGC, CSIR and other competitive exams M S Shekhawat Vikrant In Vitro Principles, Techniques and Applications