TY  - BOOK
AU  - Parys,Jan B.
AU  - Bootman,Martin D.
AU  - Yule,David I.
AU  - Bultynck,Geert
TI  - Calcium Techniques: A Laboratory Manual 
SN  - 9781621820789 (hbk.)
AV  - QP535.C2 C35 2014
U1  - 572/.516 23
PY  - 2014///
CY  - New York
PB  - Cold Spring Harbor Laboratory Press
KW  - Calcium
KW  - physiology
KW  - Laboratory Manuals
KW  - Calcium Channels
KW  - Calcium Signaling
KW  - Fluorometry
KW  - methods
N1  - Includes bibliographical references and index; Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics
N2  - "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
ER  -