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Advances in Molecular Techniques

By: Material type: TextTextLanguage: English Publication details: Boca Raton, FL : CRC Press, 2018.Edition: 1st edDescription: 436pISBN:
  • 9780815370758
Subject(s): DDC classification:
  • 572.33 SEN
Contents:
Contents: Cover; Half Title; Title Page; Copyright Page; Dedication; Contents; Foreword; Preface; Authors; 1. DNA Manipulative and Ingratiative Techniques; Isolation of Total Genomic DNA; Isolation of Plant DNA Using the CTAB Method; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Isolation of Plant DNA Using the SDS Method; Introduction; Materials Required; Method; Precautions; To Isolate Plasmid DNA from Bacteria; Introduction; Materials Required; Method; Precautions; Isolation of Chloroplasts; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation Contents: MethodResults; Precautions; Isolation of Chloroplast DNA; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Method; Results; Precautions; Isolation of Yeast Genomic DNA; Introduction; Materials Required; Method; Isolation of Bacterial Genomic DNA; Introduction; Materials Required; Method; Large-Scale Isolation of Cosmid DNA; Isolation; Purification; Miniprep Double-Stranded DNA Isolation; Principle; Isolation; Standard Alkaline Lysis Purification; Diatomaceous Earth-Base Purification; Plasmid Miniprep by Birnboim Method; Materials Required Contents: Plasmid Miniprep by Boiling MethodMaterials Required; Method; Isolation of Lambda (.) DNA; Materials Required; Method; Quick Method for isolation of Lambda (.) DNA; Materials Required; Single-Stranded M13 DNA Isolation Using Phenol; Method; Biomek-Automated Modified-Eperon Isolation Procedure for Single-Stranded M13 DNA; Method; Isolation of Aspergillus DNA; Materials Required; Method; Isolation of Aspergillus DNA by the Qiagen Method; Materials Required; Method; Purification of Genomic DNA; Introduction; Principle; Materials Required; Method; Materials Required Contents: Isolation of RNA from Plant TissuesIntroduction; Extraction of Total RNA; Materials Required; Method; Precautions; Extraction of Polysomal RNA of Nuclear Sequences; Materials Required; Chemicals; Glassware and Other; Equipment; Isolation of Total RNA form Bacterial Cells; Introduction; Materials Required; Method; Quantitative Determination of DNA and RNA by a Spectrophotometric Method; Introduction; Materials Required; Agarose Gel Electrophoresis; Introduction; Preparation of Agarose Gel; Electrophoresis of DNA Fragments; Visualization of DNA Fragments; Materials Required; Chemicals Contents: Glassware and OtherEquipment; Electrophoresis of DNA: Linear, Circular, and Super Coiled; Introduction; Experimental Outline; Materials Required; Method; Precautions; Elution of Plant DNA Fragment from Agarose Gel; Introduction; Materials Required; Method; Precautions; Hybridization and Autoradiography of DNA; Introduction; Experimental Outline; Materials Required; Method; Hybridization; Autoradiography; Precautions; PCR in Outline; Introduction; Choice Between Cloning and PCR; Experimental Outline; Materials Required; Method; Precautions
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Text Books Text Books CUTN Central Library Sciences Non-fiction 572.33 SEN (Browse shelf(Opens below)) Available 44210

Contents: Cover; Half Title; Title Page; Copyright Page; Dedication; Contents; Foreword; Preface; Authors; 1. DNA Manipulative and Ingratiative Techniques; Isolation of Total Genomic DNA; Isolation of Plant DNA Using the CTAB Method; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Isolation of Plant DNA Using the SDS Method; Introduction; Materials Required; Method; Precautions; To Isolate Plasmid DNA from Bacteria; Introduction; Materials Required; Method; Precautions; Isolation of Chloroplasts; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation
Contents: MethodResults; Precautions; Isolation of Chloroplast DNA; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Method; Results; Precautions; Isolation of Yeast Genomic DNA; Introduction; Materials Required; Method; Isolation of Bacterial Genomic DNA; Introduction; Materials Required; Method; Large-Scale Isolation of Cosmid DNA; Isolation; Purification; Miniprep Double-Stranded DNA Isolation; Principle; Isolation; Standard Alkaline Lysis Purification; Diatomaceous Earth-Base Purification; Plasmid Miniprep by Birnboim Method; Materials Required
Contents: Plasmid Miniprep by Boiling MethodMaterials Required; Method; Isolation of Lambda (.) DNA; Materials Required; Method; Quick Method for isolation of Lambda (.) DNA; Materials Required; Single-Stranded M13 DNA Isolation Using Phenol; Method; Biomek-Automated Modified-Eperon Isolation Procedure for Single-Stranded M13 DNA; Method; Isolation of Aspergillus DNA; Materials Required; Method; Isolation of Aspergillus DNA by the Qiagen Method; Materials Required; Method; Purification of Genomic DNA; Introduction; Principle; Materials Required; Method; Materials Required
Contents: Isolation of RNA from Plant TissuesIntroduction; Extraction of Total RNA; Materials Required; Method; Precautions; Extraction of Polysomal RNA of Nuclear Sequences; Materials Required; Chemicals; Glassware and Other; Equipment; Isolation of Total RNA form Bacterial Cells; Introduction; Materials Required; Method; Quantitative Determination of DNA and RNA by a Spectrophotometric Method; Introduction; Materials Required; Agarose Gel Electrophoresis; Introduction; Preparation of Agarose Gel; Electrophoresis of DNA Fragments; Visualization of DNA Fragments; Materials Required; Chemicals
Contents: Glassware and OtherEquipment; Electrophoresis of DNA: Linear, Circular, and Super Coiled; Introduction; Experimental Outline; Materials Required; Method; Precautions; Elution of Plant DNA Fragment from Agarose Gel; Introduction; Materials Required; Method; Precautions; Hybridization and Autoradiography of DNA; Introduction; Experimental Outline; Materials Required; Method; Hybridization; Autoradiography; Precautions; PCR in Outline; Introduction; Choice Between Cloning and PCR; Experimental Outline; Materials Required; Method; Precautions

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