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Antiviral : methods and protocols / edited by Edwin Yunhao Gong.

By: Contributor(s): Material type: TextTextLanguage: English Series: Methods in molecular biology (Clifton, N.J.) ; v. 1030.Publication details: New York : Humana Press ; Springer, c2013.Edition: 2nd edDescription: xiv, 452 p. : ill. (some col.) ; 26 cmISBN:
  • 9781627034838 (alk. paper)
  • 1627034838 (alk. paper)
Subject(s): DDC classification:
  • 615.7924 23 GON
Contents:
Human immunodeficiency virus type 1 (HIV-1) -- Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors / Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion / Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay / Biochemical screening assays to identify HIV-1 integrase inhibitors / HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance / Hepatitis viruses -- In vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis / Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template / Selecting and characterizing drug-resistant hepatitis C virus replicon / Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors / Expression and purification of hepatitis C virus protease from clinical samples / Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase / Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b / Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures / In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates / Flaviviruses -- Cell-based antiviral assays for screening and profiling inhibitors against dengue virus / Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes / High-throughput screening using dengue virus reporter genomes / Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate / Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase / Detection and quantification of flavivirus NS5 methyl- transferase activities / Testing antiviral compounds in a dengue mouse model / Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds / Herpes viruses -- Methods for screening and profiling inhibitors of herpes simplex viruses / In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models / Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors / Respiratory viruses -- Fluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus / Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells / Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models / In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model / Methods to determine mechanism of action of anti-influenza inhibitors / Methods for evaluation of antiviral efficacy against influenza virus infections in animal models / Alphaviruses -- Development of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell / Mouse model of chikungunya virus with utility in antiviral studies /
Part I Peggy Geluykens ... [et al.] -- Liesbet Smeulders ... [et al.] -- Bertrand Van Schoubroeck ... [et al.] -- Marleen Clynhens ... [et al.] -- Peter Van den Eede ... [et al.] -- Part II Rumin Zhang and William T. Windsor -- Eric Ferrari and Hsueh-Cheng Huang -- Inge Vliegen, Leen Delang, and Johan Neyts -- Leen Vijgen ... [et al.] -- Liesbet van der Helm -- Erna Cleiren, Benoit Devogelaere, and Katleen Fierens -- Diana Koletzki ... [et al.] -- Dawei Cai ... [et al.] -- Yang Liu and Kathryn M. Kitrinos -- Part III Edwin Yunhao Gong ... [et al.] -- Edwin Yunhao Gong ... [et al.] -- Wolfgang Fischl and Ralf Bartenschlager -- Christoph Nitsche and Christian D. Klein -- Edwin Yunhao Gong ... [et al.] -- Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi -- Wouter Schul, Andy Yip, and Pei-Yong Shi -- Sofia L. Alcaraz-Estrada, Erin Donohue Reichert, and Radhakrishnan Padmanabhan -- Part IV Edwin Yunhao Gong -- Frances Valencia, Ronald L. Veselenak, and Nigel Bourne -- Christel Van den Eynde ... [et al.] -- Part V Leen Kwanten, Ben De Clerck, and Dirk Roymans -- Anna Lundin, Tomas Bergström, and Edward Trybala -- Joke Van den Berg, Leen Kwanten, and Dirk Roymans -- Lieselot Houspie ... [et al.] -- Angela Luttick, Stephanie Hamilton, and Simon P. Tucker -- Donald F. Smee and Dale L. Barnard -- Part VI Edwin Yunhao Gong ... [et al.] -- Ashley Dagley and Justin G. Julander.
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Item type Current library Collection Call number Status Date due Barcode
General Books General Books CUTN Central Library Medicine, Technology & Management Non-fiction 615.7924 GON (Browse shelf(Opens below)) Available 37522

Human immunodeficiency virus type 1 (HIV-1) -- Fluorescence-based high-throughput screening assay to identify HIV-1 inhibitors / Homogeneous time-resolved fluorescence assay to identify inhibitors of HIV-1 fusion / Identification of HIV-1 reverse transcriptase inhibitors using a scintillation proximity assay / Biochemical screening assays to identify HIV-1 integrase inhibitors / HIV-1 genotyping of the protease-reverse transcriptase and integrase genes to detect mutations that confer antiretroviral resistance / Hepatitis viruses -- In vitro kinetic profiling of hepatitis C virus NS3 protease inhibitors by progress curve analysis / Novel hepatitis C virus NS5B polymerase assay of de novo initiated RNA synthesis directed from a heteropolymeric RNA template / Selecting and characterizing drug-resistant hepatitis C virus replicon / Cellular replicon-based phenotyping assay to determine susceptibility of hepatitis C virus clinical isolates to NS3/4A protease inhibitors / Expression and purification of hepatitis C virus protease from clinical samples / Surface plasmon resonance as a tool to select potent drug candidates for hepatitis C virus NS5B polymerase / Amplification and sequencing of the hepatitis C virus NS3/4A protease and the NS5B polymerase regions for genotypic resistance detection of clinical isolates of subtypes 1a and 1b / Southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures / In vitro phenotyping of recombinant hepatitis B virus containing the polymerase/reverse transcriptase gene from clinical isolates / Flaviviruses -- Cell-based antiviral assays for screening and profiling inhibitors against dengue virus / Duplex real-time RT-PCR assay for profiling inhibitors of four dengue serotypes / High-throughput screening using dengue virus reporter genomes / Fluorimetric and HPLC-based dengue virus protease assays using a FRET substrate / Expression and purification of dengue virus NS5 polymerase and development of a high-throughput enzymatic assay for screening inhibitors of dengue polymerase / Detection and quantification of flavivirus NS5 methyl- transferase activities / Testing antiviral compounds in a dengue mouse model / Construction of self-replicating subgenomic west nile virus replicons for screening antiviral compounds / Herpes viruses -- Methods for screening and profiling inhibitors of herpes simplex viruses / In vivo evaluation of antiviral efficacy against genital herpes using mouse and guinea pig models / Fluorescence-based high-throughput screening assay for identifying human cytomegalovirus inhibitors / Respiratory viruses -- Fluorescence-based high-throughput antiviral compound screening assay against respiratory syncytial virus / Screening and evaluation of anti-respiratory syncytial virus compounds in cultured cells / Evaluation of antiviral efficacy against human respiratory syncytial virus using cotton rat and mouse models / In vivo evaluation of antiviral compounds on respiratory syncytial virus using a juvenile vervet monkey (Chlorocebus pygerythrus) infection model / Methods to determine mechanism of action of anti-influenza inhibitors / Methods for evaluation of antiviral efficacy against influenza virus infections in animal models / Alphaviruses -- Development of a high-throughput antiviral assay for screening inhibitors of chikungunya virus and generation of drug-resistant mutations in cultured cell / Mouse model of chikungunya virus with utility in antiviral studies /

Includes bibliographical references and index.

Part I Peggy Geluykens ... [et al.] -- Liesbet Smeulders ... [et al.] -- Bertrand Van Schoubroeck ... [et al.] -- Marleen Clynhens ... [et al.] -- Peter Van den Eede ... [et al.] -- Part II Rumin Zhang and William T. Windsor -- Eric Ferrari and Hsueh-Cheng Huang -- Inge Vliegen, Leen Delang, and Johan Neyts -- Leen Vijgen ... [et al.] -- Liesbet van der Helm -- Erna Cleiren, Benoit Devogelaere, and Katleen Fierens -- Diana Koletzki ... [et al.] -- Dawei Cai ... [et al.] -- Yang Liu and Kathryn M. Kitrinos -- Part III Edwin Yunhao Gong ... [et al.] -- Edwin Yunhao Gong ... [et al.] -- Wolfgang Fischl and Ralf Bartenschlager -- Christoph Nitsche and Christian D. Klein -- Edwin Yunhao Gong ... [et al.] -- Siew Pheng Lim, Christophe Bodenreider, and Pei-Yong Shi -- Wouter Schul, Andy Yip, and Pei-Yong Shi -- Sofia L. Alcaraz-Estrada, Erin Donohue Reichert, and Radhakrishnan Padmanabhan -- Part IV Edwin Yunhao Gong -- Frances Valencia, Ronald L. Veselenak, and Nigel Bourne -- Christel Van den Eynde ... [et al.] -- Part V Leen Kwanten, Ben De Clerck, and Dirk Roymans -- Anna Lundin, Tomas Bergström, and Edward Trybala -- Joke Van den Berg, Leen Kwanten, and Dirk Roymans -- Lieselot Houspie ... [et al.] -- Angela Luttick, Stephanie Hamilton, and Simon P. Tucker -- Donald F. Smee and Dale L. Barnard -- Part VI Edwin Yunhao Gong ... [et al.] -- Ashley Dagley and Justin G. Julander.

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