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Auditory and vestibular research : methods and protocols / edited by Bernd Sokolowski.

Contributor(s): Material type: TextTextSeries: Springer protocols | Methods in molecular biology (Clifton, N.J.) ; v. 493.Publication details: New York, N.Y. : Humana ; [London : Springer, distributor], c2009.Description: xii, 421 p. : ill. (some col.) ; 27 cmISBN:
  • 9781934115626 (hbk.)
  • 1934115622 (hbk.)
  • 9781597455237 (ebk.)
  • 1597455237 (ebk.)
Subject(s): DDC classification:
  • 612.858 22 24752
LOC classification:
  • QP471 .A93 2009
NLM classification:
  • WV 270 A912 2009
Contents:
RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio ... [et al.] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande ... [et al.] -- Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn, and Stefan Heller -- Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa / Laura Nistico ... [et al.] -- Positional cloning of deafness genes / Hannie Kremer and Frans P.M. Cremers -- Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana, and Peter G. Gillespie -- Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H.A. Sokolowski -- Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan ... [et al.] -- In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H.A. Sokolowski -- Identification of transcription factor-DNA interactions using chromatin immunoprecipitation assays / Liping Nie, Ana E. Vázquez, and Ebenezer N. Yamoah -- Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan, Marian J. Drescher -- Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan -- Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith ... [et al.] -- Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada.
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Text Books Text Books CUTN Central Library Sciences 612.858 24752 (Browse shelf(Opens below)) Available 24752

Includes bibliographical references and index.

RNA isolation from Xenopus inner ear sensory endorgans for transcriptional profiling and molecular cloning / Casilda Trujillo-Provencio ... [et al.] -- Synthesis of biotin-labeled RNA for gene expression measurements using oligonucleotide arrays / Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah -- In situ hybridization approach to study mRNA expression and distribution in cochlear frozen sections / Hakim Hiel -- Lineage analysis of inner ear cells using genomic tags for clonal identification / Takunori Satoh and Donna M. Fekete -- Genetic fate-mapping approaches : new means to explore the embryonic origins of the cochlear nucleus / Jun Chul Kim and Susan M. Dymecki -- The practical use of Cre and loxP technologies in mouse auditory research / Yiling Yu and Jian Zuo -- Helios gene gun-mediated transfection of the inner ear sensory epithelium / Inna A. Belyantseva -- Electroporation-mediated gene transfer to the developing mouse inner ear / John V. Brigande ... [et al.] -- Isolation of sphere-forming stem cells from the mouse inner ear / Kazuo Oshima, Pascal Senn, and Stefan Heller -- Molecular biology of vestibular schwannomas / Long-Sheng Chang and D. Bradley Welling -- Multilocus sequence typing and pulsed field gel electrophoresis of otitis media causing pathogens / Jonathan C. Thomas and Melinda M. Pettigrew -- Fluorescence "in situ" hybridization for the detection of biofilm in the middle ear and upper respiratory tract mucosa / Laura Nistico ... [et al.] -- Positional cloning of deafness genes / Hannie Kremer and Frans P.M. Cremers -- Twist-off purification of hair bundles / Jung-Bum Shin, James Pagana, and Peter G. Gillespie -- Yeast two-hybrid screening to test for protein-protein interactions in the auditory system / Dhasakumar S. Navaratnam -- The use of 2-D gels to identify novel protein-protein interactions in the cochlea / Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H.A. Sokolowski -- Identification of functionally important residues/domains in membrane proteins using an evolutionary approach coupled with systematic mutational analysis / Lavanya Rajagopalan ... [et al.] -- In vivo verification of protein interactions in the inner ear by coimmunoprecipitation / Margaret C. Harvey and Bernd H.A. Sokolowski -- Identification of transcription factor-DNA interactions using chromatin immunoprecipitation assays / Liping Nie, Ana E. Vázquez, and Ebenezer N. Yamoah -- Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia / Dennis G. Drescher, Neeliyath A. Ramakrishnan, Marian J. Drescher -- Multiplexed isobaric tagging protocols for quantitative mass spectrometry approaches to auditory research / Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan -- Fluorescence microscopy methods in the study of protein structure and function / Heather Jensen-Smith ... [et al.] -- Ion imaging in the cochlear hair cells / Gregory I. Frolenkov -- Atomic force microscopy in studies of the cochlea / Michio Murakoshi and Hiroshi Wada.

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