000			06671cam a22005777a 4500
999			_c30339 _d30339
003			CUTN
005			20190827152919.0
008			130307s2013 nyua b 001 0 eng
020			_a9781627034005
020			_a1627034005
020			_z9781627034012
041			_aEnglish
082	0	4	_a571.74 _223 _bMUN
100			_aMunnik, Teun,
245	0	0	_aPlant Lipid Signaling Protocols / _cTeun Munnik & Ingo Heilmann.
260			_aNew York : _bHumana Press, _c2013.
300			_axi, 305 p. : _bill. ; _c27 cm.
440			_aMethods in molecular biology (Clifton, N.J.), _vV-1009.
500			_aElectronic books Laboratory manuals
505	0	0	_tPART :1 Analyzing plant signaling phospholipids through 32Pi-labeling and TLC / _tPART :2 Analysis of D3-,4-,5-phosphorylated phosphoinositides using HPLC / _tPART :3 Mass measurement of polyphosphoinositides by thin-layer and gas chromatography / _tPART :4 Measurement of inositol (1,4,5) trisphosphate in plant tissues by a competitive receptor binding assay / _tPART :5 Quantification of diacylglycerol by mass spectrometry / _tPART :6 Distinguishing phosphatidic acid pools from de novo synthesis, PLD, and DGK / _tPART :7 Use of phospholipase A2 for the production of lysophospholipids / _tPART :8 Analysis and quantification of plant membrane lipids by thin-layer chromatography and gas chromatography / _tPART :9 Lipidomic analysis of plant membrane lipids by direct infusion tandem mass spectrometry / _tPART :10 Detection and quantification of plant sphingolipids by LC-MS / _tPART :11 Analysis of defense signals in Arabidopsis thaliana leaves by ultra-performance liquid chromatography/tandem mass Spectrometry : jasmonates, salicylic acid, abscisic acid / _tPART :12 Analysis of fatty acid amide hydrolase activity in plants / _tPART :13 Ionization behavior of polyphosphoinositides determined via the preparation of pH titration curves using solid-state 31P NMR / _tPART :14 Phosphatidylinositol synthase activity from plant membrane fractions and from E. coli- expressed recombinant peptides / _tPART :15 Phosphatidylinositol 4-kinase and phosphatidylinositol 4-phosphate 5-kinase assays / _tPART :16 Assaying inositol and phosphoinositide phosphatase enzymes / _tPART :17 Determination of phospholipase C activity in vitro / _tPART :18 Assaying nonspecific phospholipase C activity / _tPART :19 Assaying different types of plant phospholipase D activities in vitro / _tPART :20 Measuring PLD activity in vivo / _tPART :21 Assaying plant phosphatidic acid phosphatase activity / _tPART :22 Assay of phospholipase A activity / _tPART :23 Lipid-binding analysis using a fat blot assay / _tPART :24 Liposome-binding assays to assess specificity and affinity of phospholipid-protein interactions / _tPART :25 Lipid affinity beads : from identifying new lipid binding proteins to assessing their binding properties / _tPART :26 Using genetically encoded fluorescent reporters to image lipid signalling in living plants / _tPART :27 Imaging changes in cytoplasmic calcium using the yellow cameleon 3.6 biosensor and confocal microscopy /
520			_a As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals
650		0	_aPlant cellular signal transduction
650		0	_aPlant lipids
650	1	2	_aLipids
650	2	2	_aLipid Metabolism
650	2	2	_aPlants
650		7	_aPlant cellular signal transduction.
650		7	_aPlant lipids.
700	1		_aHeilmann, Ingo,
942			_2ddc _cBOOKS
490	1		_aMethods in molecular biology, _x1064-3745 ; _v1009
490	1		_aSpringer protocols, _x1949-2448
504			_aIncludes bibliographical references and index.
505	0	0	_rTeun Munnik and Xavier Zarza -- _rTeun Munnik -- _rMareike Heilmann and Ingo Heilmann -- _rIngo Heilmann and Imara Y. Perera -- _rKatharina vom Dorp, Isabel Dombrink, and Peter Dörmann -- _rSteven A. Arisz and Teun Munnik -- _rSteven A. Arisz and Teun Munnik -- _rVera Wewer, Peter Dörmann, and Georg Hölzl -- _rSunitha Shiva ... [et al.] -- _rJonathan E. Markham -- _rNadja Stingl ... [et al.] -- _rSang-Chul Kim, Lionel Faure, and Kent D. Chapman -- _rZachary T. Graber and Edgar E. Kooijman -- _rSylvie Collin and Françoise Cochet -- _rYang Ju Im ... [et al.] -- _rJanet L. Donahue, Mustafa Ercetin, and Glenda E. Gillaspy -- _rS.M. Teresa Hernández-Sotomayor and J. Armando Muñoz-Sanchez -- _rP řemysl Pejchar, Günther F.E. Scherer, and Jan Martinec -- _rKirk L. Pappan and Xuemin Wang -- _rTeun Munnik and Ana M. Laxalt -- _rYuki Nakamura -- _rMichael Heinze and Werner Roos -- _rTeun Munnik and Magdalena Wierzchowiecka -- _rMagdalena M. Julkowska, Johanna M. Rankenberg, and Christa Testerink -- _rFionn McLoughlin and Christa Testerink -- _rJoop E.M. Vermeer and Teun Munnik -- _rSarah J. Swanson and Simon Gilroy.
650		0	_vLaboratory manuals.
650		0	_vLaboratory manuals.
650	1	2	_xbiosynthesis _vLaboratory Manuals.
650	2	2	_vLaboratory Manuals.
650	2	2	_xchemistry _vLaboratory Manuals.
650		7	_2fast
650		7	_2fast
653		4	_aLife sciences.
653		4	_aBotany.
653		4	_aPlant Sciences.
655		7	_aHandbooks, manuals, etc. _2fast
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 1009.
830		0	_aSpringer protocols (Series)
906			_a7 _bcbc _ccopycat _d2 _eepcn _f20 _gy-gencatlg