000			03478nam a22002657a 4500
999			_c32620 _d32620
003			CUTN
005			20200910142953.0
008			200910b ||||| |||| 00| 0 eng d
020			_a9788181287656
041			_aEnglish
082			_222 _a660.6 _bROS
100			_aRosenthal, Sandra J.
245			_aNanobiotechnology Protocols / _cedited by Sandra J. Rosenthal and David W. Wright.
260			_aTotowa, N. J. : _bHumana Press, _cc2005.
300			_axii, 228 p. : _bill. ; _c24 cm.
440			_aMethods in molecular biology ; _v303
500			_aIncludes bibliographical references and index.
505			_tApplications of quantum dots in biology: an overview _tFluoroimmunoassays using antibody-conjugated quantum dots _tLabeling cell-surface proteins via antibody quantum dot streptavidin conjugates _tPeptide-conjugated quantum dots: imaging the angiotensin type 1 receptor in living cells _tQuantum dot-encoded beads _tUse of nanobarcodes particles in bioassays _tAssembly and characterization of biomolecule-gold nanoparticle conjugates and their use in intracellular imaging _tWhole-blood immunoassay facilitated by gold nanoshell-conjugate antibodies _tAssays for selection of single-chain fragment variable recombinant antibodies to metal nanoclusters _tSurface-functionalized nanoparticles for controlled drug delivery _tScreening of combinatorial peptide libraries for nanocluster synthesis _tStructural DNA nanotechnology: an overview _tNanostructured DNA templates _tSynthetic nanoscale elements for delivery of materials into viable cells _tReal-time cell dynamics with a multianalyte physiometer.
520			_aThe combination of nanoscience and biotechnology promises to yield revolutionary biological insights, ranging from receptor function to drug discovery to personal medicine. In NanoBiotechnology Protocols, hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The authors emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used as a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotechnology arena. A value-added compact disk containing color figures is included. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Illuminating and cross-disciplinary, NanoBiotechnology Protocols enables novice and experienced researchers alike to quickly come up to speed with both nanomaterials preparation and the use of nanomaterials in biological and medicinal applications.
650			_aNanobiotechnology
650			_aBiotechnology.
650			_aNanotechnology.
700			_aWright, David W.
942			_2ddc _cBOOKS