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020 _a9781071616475
041 _aEnglish
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082 0 4 _a581.35
_223
_bVAS
245 1 0 _aPlant Circular RNAs :
_bMethods and Protocols /
_cedited by Luis María Vaschetto.
250 _a1st ed. 2021.
260 _aNew York, NY:
_bSpringer US
_c2021.
300 _aX, 196 p. 43 illus.
490 1 _aMethods in Molecular Biology,
_x1940-6029 ;
_v2362.
505 0 _aIdentification and Characterization of Plant Circular RNAs -- Experimental Strategies for Studying the Function of Plant circRNAs -- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization -- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development -- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing -- Identification and Functional Characterization of Viroid Circular RNAs -- Circular RNA Databases -- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs -- Computational Analysis of Transposable Elements and circRNAs in Plants -- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database -- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.
520 _aThis volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs' research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field. .
650 0 _aPlant genetics.
650 0 _aGenetics.
650 0 _aBioinformatics.
700 1 _aVaschetto, Luis María.
700 1 _eeditor.
710 2 _aSpringerLink (Online service)
830 0 _aMethods in Molecular Biology,
_v2362.
856 4 0 _uhttps://ezproxy.lib.gla.ac.uk/login?url=https://doi.org/10.1007/978-1-0716-1645-1
856 4 0 _zConnect to e-book
907 _a.b38107661
942 _2ddc
_cBOOKS
999 _c44338
_d44338