000			01748nam a22001937a 4500
003			CUTN
005			20250602162759.0
008			250602b |||||||| |||| 00| 0 eng d
020			_a9783662668535
041			_aEnglish
082			_223 _a572.8 _bSUR
100			_aSurzycki, Stefan
245			_aBasic techniques in molecular biology / _cStefan Surzycki
260			_aNew Delhi : _bSpringer India, _c2000.
300			_a434 p. : _bIll. ; _c25 cm
520			_aBasic Techniques in Molecular Biology by Stefan Surzycki is a comprehensive, practical guide designed for students and researchers entering the field of molecular biology. The book systematically covers the fundamental laboratory techniques essential for working with nucleic acids and proteins. It begins with an introduction to the core concepts of molecular biology and crucial laboratory safety and sterile techniques. The text then delves into detailed explanations of nucleic acid extraction and purification, restriction enzyme digestion, and gel electrophoresis. A significant portion is dedicated to the Polymerase Chain Reaction (PCR), including its variations and troubleshooting, and the intricate process of gene cloning and recombinant DNA technology. Later chapters explore DNA sequencing methodologies and various protein-related techniques such as extraction, SDS-PAGE, and Western blotting. The book also introduces the basics of bioinformatics, emphasizing the use of databases and sequence analysis tools. Throughout, Surzycki focuses on practical application, experimental design, and troubleshooting, making it an invaluable resource for anyone seeking a solid foundation in molecular biology laboratory work.
650			_aMolecular Biology
942			_2ddc _cBOOKS
999			_c44562 _d44562