TY - BOOK TI - An Introduction to Biotechnology: The Science, Technology and Medical Applications SN - 9789351073161 U1 - 660.6 1st PY - 0000///152014/// CY - New Delhi PB - Academic Press N1 - List of Figures ; List of Tables ; Preface ; About the Author ; Chapter 1: Membranes Abstract 1.1 Membrane Lipids 1.2 Cholesterol 1.3 Membrane Proteins Questions; Chapter 2: Proteins Abstract 2.1 Amino Acids 2.2 Protein Structure 2.3 The Hydrophobic Effect 2.4 A Return to Membranes Questions ; Chapter 3: Cellular Transport Abstract 3.1 Membrane Transporters 3.2 Vesicular Transporters: Endocytosis 3.3 Receptor Fates 3.4 Lysosomes Are for Degradation, But Are They Safe? Questions ; Chapter 4: Genes: The Blueprints for Proteins Abstract 4.1 Nucleotides and Nucleic Acids 4.2 From Genes to Proteins Questions ; Chapter 5: Cell Growth Abstract 5.1 The Eukaryotic Cell Cycle 5.2 Growth Curves and Their Phases 5.3 Mathematics of the Growth Curve 5.4 Counting Cell Numbers 5.5 Counting Cell Mass 5.6 Scale-Up Questions ; Chapter 6: Microbial Killing Abstract 6.1 The Gram Stain 6.2 Microbial Resistance to Killing 6.3 Sterilization, Disinfection, and Sanitization 6.4 Microbial Cell Death Questions ; Chapter 7: Cell Culture and the Eukaryotic Cells Used in Biotechnology Abstract 7.1 Adherent Cells Versus Nonadherent Cells 7.2 Primary Cells, Cancer Cells, and Cell Lines Questions ; Chapter 8: Fluorescence Abstract 8.1 Stokes’ Experiments 8.2 Fluorophore Properties 8.3 Fluorescence Detection 8.4 FRET Questions ; Chapter 9: Locating Transcriptional Control Regions: Deletion Analysis Abstract 9.1 An Example of Deletion Analysis Questions ; Chapter 10: Agarose Gels Abstract 10.1 Application of Agarose Gels: Gel Shift 10.2 Application of Agarose Gels: DNA Footprinting 10.3 Application of Agarose Gels: Restriction Analysis Questions ; Chapter 11: The Polymerase Chain Reaction Abstract 11.1 Melt 11.2 Anneal 11.3 Extend 11.4 PCR Loops 11.5 An Application of Traditional PCR 11.6 Traditional Versus Real-Time PCR 11.7 Real-Time PCR Questions ; Chapter 12: Genetic Engineering Abstract 12.1 Plasmid Architecture 12.2 Molecular Cloning 12.3 A Single Plasmid Is Not Enough 12.4 Spectrophotometry 12.5 What We Have Learned so Far Questions ; Chapter 13: Gene Delivery Abstract 13.1 Gene Delivery Vehicles: An Overview 13.2 Gene Methods in Greater Detail 13.3 Preparation of Nonviral Gene Delivery Complexes Questions ; Chapter 14: RNAi Abstract 14.1 Cosuppression 14.2 RNA Interference 14.3 miRNA Questions ; Chapter 15: DNA Fingerprinting Abstract 15.1 Older DNA Fingerprinting Uses RFLPs 15.2 Newer DNA Fingerprinting Uses STRs Questions Chapter 16: Fermentation, Beer, and Biofuels Abstract 16.1 Glycolysis 16.2 Fermentation 16.3 The Production of Beer 16.4 Fermentation to Produce Biofuels Questions Chapter 17: Stem Cells and Tissue Engineering Abstract 17.1 Potential 17.2 An Alternate View of Stem Cells 17.3 Using Stem Cells 17.4 Tissue Engineering and Regenerative Medicine 17.5 Bioreactors 17.6 Polymeric Scaffolds 17.7 Bringing it all Together: A Tissue Engineering Application Questions ; Chapter 18: Transgenics Abstract 18.1 Ice-Minus Bacteria 18.2 Bt Plants 18.3 Herbicide Resistance 18.4 Tomatoes 18.5 Rice 18.6 Terminators and Traitors Questions ; Chapter 19: Patents and Licenses Abstract 19.1 Types of Patents 19.2 Licenses 19.3 After a License Is Granted Questions ; Index ER -