Includes bibliographical references and index.

Ruifeng He -- Pooja Saxena [and others] -- Qiang Chen [and others] -- Daniel Guzmán-Zapata [and others] -- Nicoletta Ferradini [and others] -- Alexandra Castilho and Herta Steinkellner -- Stéphanie Robert [and others] -- Lauren Moore, Krystal Hamorsky, and Nobuyuki Matoba -- Manish L. Raorane, Joan O. Narciso, and Ajay Kohli -- Sherwin Barretto, Franck Michoux, and Peter J. Nixon -- Nicole Raven, Stefan Schillberg, and Stefan Rasche -- Cory L. Nykiforuk -- Lauri J. Reuter, Andrew J. Conley, and Jussi J. Joensuu -- Kurniawan Rudi Trijatmiko [and others] -- Yuhya Wakasa and Fumio Takaiwa -- Kazumi Kitta, Reona Takabatake, and Junichi Mano -- Gerson Graser and Frederick S. Walters -- Dieter Peschen, Stefan Schillberg, and Rainer Fischer.

This volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production. Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.