Microbiological Techniques is designed for the students, to explore the world of microorganisms and how the process of scientific discovery is carried out, with an ease. The book primarily focuses on the basic microbiological techniques with applications for undergraduate and postgraduate students in diverse area of biological techniques. This book is the outcome of nearly a decade of teaching and research experience. The manual comprises twelve parts in which exercises in first three parts provide sequential developments of fundamental techniques. The remaining exercises are as independent as possible to allow the instructor to select the desirable sequence. The style and simplicity of expression have been our twin objectives. All exercises have been thoroughly tested in our laboratory by our students with wide variety of real talents and enthusiasm.