Basic Techniques in Molecular Biology by Stefan Surzycki is a comprehensive, practical guide designed for students and researchers entering the field of molecular biology. The book systematically covers the fundamental laboratory techniques essential for working with nucleic acids and proteins. It begins with an introduction to the core concepts of molecular biology and crucial laboratory safety and sterile techniques. The text then delves into detailed explanations of nucleic acid extraction and purification, restriction enzyme digestion, and gel electrophoresis. A significant portion is dedicated to the Polymerase Chain Reaction (PCR), including its variations and troubleshooting, and the intricate process of gene cloning and recombinant DNA technology. Later chapters explore DNA sequencing methodologies and various protein-related techniques such as extraction, SDS-PAGE, and Western blotting. The book also introduces the basics of bioinformatics, emphasizing the use of databases and sequence analysis tools. Throughout, Surzycki focuses on practical application, experimental design, and troubleshooting, making it an invaluable resource for anyone seeking a solid foundation in molecular biology laboratory work.