Calcium Techniques :
Calcium Techniques : A Laboratory Manual /
edited by Jan B. Parys, KU Leuven, Martin D. Bootman, The Open University, David I. Yule, University of Rochester, Geert Bultynck, KU Leuven.
- New York ; Cold Spring Harbor Laboratory Press, c2014.
- xvi, 592 pages : illustrations (some color) ; 28 cm
Includes bibliographical references and index.
Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.
"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
9781621820789 (hbk.) 1621820785 (hbk.) 9781936113583 (pbk.) 1936113589 (pbk.)
2013024333
Calcium--physiology--Laboratory Manuals.
Calcium Channels--Laboratory Manuals.
Calcium Signaling--Laboratory Manuals.
Fluorometry--methods--Laboratory Manuals.
QP535.C2 / C35 2014
572/.516
QV 25
Includes bibliographical references and index.
Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.
"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
9781621820789 (hbk.) 1621820785 (hbk.) 9781936113583 (pbk.) 1936113589 (pbk.)
2013024333
Calcium--physiology--Laboratory Manuals.
Calcium Channels--Laboratory Manuals.
Calcium Signaling--Laboratory Manuals.
Fluorometry--methods--Laboratory Manuals.
QP535.C2 / C35 2014
572/.516
QV 25