Introduction to instrumentation in life sciences /
Bisen, Prakash S.
Introduction to instrumentation in life sciences / Prakash S. Bisen, Anjana Sharma. - Boca Raton : CRC Press, 2013. - xix, 355 p. : ill. (some col.) ; 26 cm
Includes bibliographical references and index.
Microscopy
Introduction
Magnification, Resolution, and Contrast
Light (Bright Field) Microscopy
Dark Field Microscope
Phase Contrast Microscope
Interference Microscope
UV and Fluorescence Microscopes
Electron Microscopy
Tunneling Electron Microscopy
Confocal Microscopy
Techniques in Microscopy
Electron Microscopy
Suggested Readings
Important Links
Micrometry
Introduction
Structure of an Ocular Micrometer
Conjugate Image-Forming Focal Planes
Eyepiece Designs
Stage Micrometer
Filar Eyepiece Micrometer
Principle of Filar Eyepiece Micrometer
Working
Suggested Reading
Important Links
Electrochemical Techniques
Introduction
Structure of a pH Meter
Principles
Factors Limiting the Accuracy of pH Measurements
Measurement of pH
Working
Procedure for Measuring pH Using a pH Meter
Cleaning and Troubleshooting of Glass Probes
Application of pH Measurements
Oxygen Electrode
Suggested Readings
Important Links
Chromatography
Introduction
General Principles
Column Chromatography
Paper Chromatography and Thin-Layer Chromatography
Sample Collection, Preservation, and Preparation
Adsorption Chromatography
Partition Chromatography
High-Performance Liquid Chromatography
Fast Protein Liquid Chromatography
Gas–Liquid Chromatography
Ion-Exchange Chromatography
Exclusion Chromatography
Affinity Chromatography
Suggested Readings
Important Links
Spectroscopy
Introduction
UV–Visible Spectroscopy
IR (Vibrational) Spectroscopy
Flame/Atomic Absorption Spectroscopy
Fluorescence Spectroscopy
ESR Spectroscopy
NMR Spectroscopy
MALDI-TOF Mass Spectrometry
Circular Dichroism (CD) Spectroscopy
Suggested Readings
Important Links
Centrifugation
Introduction
Principle of Centrifugation
Types of Centrifuges
Types of Centrifugal Separations
Rotor Categories
Selection of Centrifuge Tubes
Common Centrifugation Vocabulary and Formulas
Analytical Ultracentrifuge
Suggested Reading
Important Links
Electrophoresis
Introduction
Principles of GE
Working with the Electrophoresis Apparatus
Denaturing Gradient GE
Temperature Gradient GE
Pulsed-Field GE
Capillary Electrophoresis
Suggested Readings
Important Links
X-Ray Microanalysis
Introduction
Principles
Instrumentation
Applications of X-Ray Microanalysis
Techniques for the Analysis of Secondary, Tertiary, and Quaternary Proteins by X-Ray Crystallography
Suggested Readings
Important Link
Techniques with Radioisotopes
Introduction
Isotopes and Radioactivity
Ionization Effects
Measurement Units
Measurement Techniques
Autoradiography
Counting Statistics
Biological Uses of Radioisotopes
Tracer Dilution Technique
Radioimmunoassay
Miscellaneous Uses of Radioisotopes
Radioisotope Production
Most Widely Used Radioisotopes
Suggested Reading
Important Links
Fermentation
Introduction
History
Principles of Fermentation
Introduction to Microorganisms
Types of Fermentation Metabolism
Fermentation Process
Types of Fermentation
Types of Fermentors
Sterilization in Fermentation
Product Recovery
Suggested Readings
Important Links
Conductivity Meter
Introduction
Principles
Common Definitions
Conductivity Meter
Conductivity Cells
Factors Influencing the Measurement
Measuring Techniques
Reliable Measurements
Maintenance and Storage
Applications
Suggested Readings
Important Links
Glossary
Index
"Designed to serve a multitude section of students and researchers diversified in the field of Life Sciences, Pharmacy, Biotechnology, Microbiology, Biochemistry, and Environmental Sciences, this book addresses the different aspects of instrumentation that hold the key for cutting edge research and innovative application. The authors emphasize techniques encountered both in the practical classes and also in high through put techniques involved in modern era of commercialization and product inventories. It includes illustrated diagrams to explain the principles and theories behind these instruments"--Provided by publisher.
9781466512405 (pbk. : alk. paper)
2012025562
Biological apparatus and supplies.
Life sciences--Research--Equipment and supplies.
Biology--instrumentation.
QH324.42 / .B57 2013
570.284 / BIS
QH 324.42
Introduction to instrumentation in life sciences / Prakash S. Bisen, Anjana Sharma. - Boca Raton : CRC Press, 2013. - xix, 355 p. : ill. (some col.) ; 26 cm
Includes bibliographical references and index.
Microscopy
Introduction
Magnification, Resolution, and Contrast
Light (Bright Field) Microscopy
Dark Field Microscope
Phase Contrast Microscope
Interference Microscope
UV and Fluorescence Microscopes
Electron Microscopy
Tunneling Electron Microscopy
Confocal Microscopy
Techniques in Microscopy
Electron Microscopy
Suggested Readings
Important Links
Micrometry
Introduction
Structure of an Ocular Micrometer
Conjugate Image-Forming Focal Planes
Eyepiece Designs
Stage Micrometer
Filar Eyepiece Micrometer
Principle of Filar Eyepiece Micrometer
Working
Suggested Reading
Important Links
Electrochemical Techniques
Introduction
Structure of a pH Meter
Principles
Factors Limiting the Accuracy of pH Measurements
Measurement of pH
Working
Procedure for Measuring pH Using a pH Meter
Cleaning and Troubleshooting of Glass Probes
Application of pH Measurements
Oxygen Electrode
Suggested Readings
Important Links
Chromatography
Introduction
General Principles
Column Chromatography
Paper Chromatography and Thin-Layer Chromatography
Sample Collection, Preservation, and Preparation
Adsorption Chromatography
Partition Chromatography
High-Performance Liquid Chromatography
Fast Protein Liquid Chromatography
Gas–Liquid Chromatography
Ion-Exchange Chromatography
Exclusion Chromatography
Affinity Chromatography
Suggested Readings
Important Links
Spectroscopy
Introduction
UV–Visible Spectroscopy
IR (Vibrational) Spectroscopy
Flame/Atomic Absorption Spectroscopy
Fluorescence Spectroscopy
ESR Spectroscopy
NMR Spectroscopy
MALDI-TOF Mass Spectrometry
Circular Dichroism (CD) Spectroscopy
Suggested Readings
Important Links
Centrifugation
Introduction
Principle of Centrifugation
Types of Centrifuges
Types of Centrifugal Separations
Rotor Categories
Selection of Centrifuge Tubes
Common Centrifugation Vocabulary and Formulas
Analytical Ultracentrifuge
Suggested Reading
Important Links
Electrophoresis
Introduction
Principles of GE
Working with the Electrophoresis Apparatus
Denaturing Gradient GE
Temperature Gradient GE
Pulsed-Field GE
Capillary Electrophoresis
Suggested Readings
Important Links
X-Ray Microanalysis
Introduction
Principles
Instrumentation
Applications of X-Ray Microanalysis
Techniques for the Analysis of Secondary, Tertiary, and Quaternary Proteins by X-Ray Crystallography
Suggested Readings
Important Link
Techniques with Radioisotopes
Introduction
Isotopes and Radioactivity
Ionization Effects
Measurement Units
Measurement Techniques
Autoradiography
Counting Statistics
Biological Uses of Radioisotopes
Tracer Dilution Technique
Radioimmunoassay
Miscellaneous Uses of Radioisotopes
Radioisotope Production
Most Widely Used Radioisotopes
Suggested Reading
Important Links
Fermentation
Introduction
History
Principles of Fermentation
Introduction to Microorganisms
Types of Fermentation Metabolism
Fermentation Process
Types of Fermentation
Types of Fermentors
Sterilization in Fermentation
Product Recovery
Suggested Readings
Important Links
Conductivity Meter
Introduction
Principles
Common Definitions
Conductivity Meter
Conductivity Cells
Factors Influencing the Measurement
Measuring Techniques
Reliable Measurements
Maintenance and Storage
Applications
Suggested Readings
Important Links
Glossary
Index
"Designed to serve a multitude section of students and researchers diversified in the field of Life Sciences, Pharmacy, Biotechnology, Microbiology, Biochemistry, and Environmental Sciences, this book addresses the different aspects of instrumentation that hold the key for cutting edge research and innovative application. The authors emphasize techniques encountered both in the practical classes and also in high through put techniques involved in modern era of commercialization and product inventories. It includes illustrated diagrams to explain the principles and theories behind these instruments"--Provided by publisher.
9781466512405 (pbk. : alk. paper)
2012025562
Biological apparatus and supplies.
Life sciences--Research--Equipment and supplies.
Biology--instrumentation.
QH324.42 / .B57 2013
570.284 / BIS
QH 324.42