Biophotonics : science and technology /
Yeh, Yin, 1938-
Biophotonics : science and technology / Yin Yeh, V. V. Krishnan. - World Scientific, © 2018. - xiii, 275 pages : illustrations (some color) ; 24 cm
Introduction to biophotonics -- Review of electromagnetic field interaction with matter -- Molecular and cellular structure -- The expanse of biology problems in need of quantitative solution -- Optical microscopy -- Label-free high-resolution microscopy -- Temporal dynamics -- Photonics in medicine. Cover Page
Halftitle
Title
Copyright
Contents
Preface
1. Introduction to Biophotonics
1.1 Definition of Biophotonics
1.2 What are Photonic Processes?
1.3 Fundamental Biology Studies Need Photonics
1.4 Applied Biology — Molecular Medicine Using Photonic Means
1.5 Layout of This Volume
2. Review of Electromagnetic Field Interaction with Matter
2.1 Electromagnetic Field, Intensity, and Photon Numbers
2.2 Classical or Quantum Mechanics Description of Interaction?
2.3 The Radiation Field and Its Quantum Description
2.4 One-photon Matter Interaction
2.4.1 Linear absorption
2.4.2 Birefringence and dichroism
2.4.3 Circular dichroism and rotatory dispersion
2.4.4 Attenuated total reflection (ATR) and totally internal reflection (TIR) spectroscopy
2.4.5 Surface plasmon resonance (SPR)
2.5 Two-photon Matter Interactions
2.5.1 Scattering of light — Structural determination and diffraction
2.5.2 Temporal studies: Dynamic Light Scattering (DLS) and Photon Correlation Spectroscopy (PCS)
2.5.3 Raman scattering (classical perspective)
2.5.4 Fluorescence spectroscopy
2.5.5 Fluorescence Resonant Energy Transfer (FRET) analysis
2.6 Nonlinear Optical Susceptibility
2.6.1 Second-order nonlinearities
2.6.2 Four-wave mixing processes
2.7 Gradient Radiation Pressure ⇒ Optical Trapping
3. Molecular and Cellular Structure
3.1 From Atoms to Molecules
3.1.1 Molecular bonding
3.1.2 Hybridization and the unique carbon bonding capability
3.1.3 Other molecular bonding mechanisms
3.2 Molecules of the Cell
3.2.1 Nucleic acids
3.2.2 Lipids
3.2.3 Proteins
3.2.4 Carbohydrates
4. The Expanse of Biology Problems in Need of Quantitative Solution
4.1 The Need to Measure at Increasingly Higher Spatial Resolution
4.1.1 X-ray diffraction and imaging
4.1.2 Electron microscopy
4.1.3 Nuclear Magnetic Resonance (NMR) spectroscopy
4.1.4 Atomic Force Microscopy (AFM) and Near-field Scanning Optical Microscopy (NSOM)
4.1.5 Optical imaging
4.2 Examples of How Optical Methods Are Used in Relating Structure to Function
4.2.1 Protection, regulation, and specification of proper functions within the cell
4.2.2 Muscle contractility
4.2.3 Genetic management: Replication, search, repair, and damage control
4.2.4 Helicase activity in prokaryotic cells
4.2.5 RNA polymerase-II
4.2.6 Telomere and telomerase
5. Optical Microscopy
5.1 Basics of an Optical Microscope
5.1.1 Summary of the needed parameters for imaging cellular or molecular parameters
5.2 Attempts to Meet the Needs of Higher Clarity in Microscopy
5.2.1 Phase contrast microscopy
5.2.2 Early X-ray microscopy imaging
5.2.3 Confocal microscopy — Beating that Abbe limit #1
5.2.4 Near-field microscopes — Beating Abbe limit #2
5.3 Using Contrast Agents, Intrinsic or Otherwise
5.3.1 Fluorescence or phosphorescence emission enhances contrast
5.3.2 Fluorescent proteins
5.3.3 Quantum dots
5.3.4 Search for better emitters
5.4 Ultrahigh Resolution Fluorescence Microscopy
5.4.1 Stimulated Emission Depletion (STED)
5.4.2 Photo-Activated Localization Microscopy (PALM) and STochastic Optical Reconstruction Microscopy (STORM)
5.4.3 Structured Illumination Microscopy (SIM)
5.5 Multiphoton Excitation Fluorescence
5.6 Summary
6. Label-free High-resolution Microscopy
6.1 Many Faces of Nonlinear Interaction
6.1.1 Second harmonic generation (SHG) and imaging
6.1.2 Sum frequency generation — Possibly spectral imaging
6.1.3 Stimulated Raman Scattering
6.1.4 Coherent Anti-Stokes Raman Scattering (CARS)
6.1.5 Self-focusing and self-phase modulation
6.2 X-ray Microscopy
6.2.1 Early soft X-ray microscopes
6.2.2 Coherent X-ray laser sources for microscopy
7. Temporal Dynamics
7.1 Particle Tracking
7.1.1 Tracking motor molecule dynamics
7.2 Time Correlation Analysis
7.2.1 Photon Correlation Spectroscopy (PCS or Dynamic Light Scattering [DLS])
7.2.2 Fluorescence Correlation Spectroscopy (FCS)
7.3 Progress in the Extension of the FCS Method
7.4 Fluorescence Cross-Correlation Spectroscopy (FCCS)
7.5 Combining Techniques
7.5.1 Multi (or Two) Photon Excited Fluorescence (M(T)PEF) FCS
7.5.2 Evanescent wave-FCS
7.6 Anti-bunching Spectroscopy
7.7 Imaging FCS
7.8 Fluorescence Resonant Energy Transfer (FRET) FCS
7.9 Summary
8. Photonics in Medicine
8.1 Visualization and Validation
8.1.1 Imaging with microscopy and endoscopy
8.1.2 Optical Coherence Tomography (OCT)
8.1.3 Optical endoscope
8.1.4 Adaptive optics microscopy
8.2 Biomarkers
8.2.1 Use of the microRNA detectors
8.2.2 From antibodies to SHALs and aptamers
8.2.3 Multiplexed microbead assays — xMAP technology
8.2.4 DNA microarrays
8.2.5 Nanoparticles
8.2.6 Fluorescence Lifetime Imaging Microscopy (FLIM)
8.3 Photonics Means of Medical Therapy
8.3.1 Photodynamic therapy (PDT)
8.3.2 Mechanism of PDT
8.3.3 Emerging areas of research in PDT
8.4 Controlling Molecular Gating Functions
8.4.1 Mechanism of action
8.5 Final Thoughts
Index
Biophotonics: Science and Technology
This lecture volume aims to give students and researchers in this rapidly expanding field of biophotonics an interdisciplinary perspective. Among the primary topics are ultrahigh resolution microscopy, particle tracking, photon correlation spectroscopy, and nonlinear optical methods as used in biological and biomedical research, with a focus on current applications in biophysics and biomedicine.
9789813235687 (hardcover)
Optical Imaging
Molecular Imaging
Microscopy
Optics and Photonics--methods--methods--methods--methods
616.075 / YEH
WN 195
Biophotonics : science and technology / Yin Yeh, V. V. Krishnan. - World Scientific, © 2018. - xiii, 275 pages : illustrations (some color) ; 24 cm
Introduction to biophotonics -- Review of electromagnetic field interaction with matter -- Molecular and cellular structure -- The expanse of biology problems in need of quantitative solution -- Optical microscopy -- Label-free high-resolution microscopy -- Temporal dynamics -- Photonics in medicine. Cover Page
Halftitle
Title
Copyright
Contents
Preface
1. Introduction to Biophotonics
1.1 Definition of Biophotonics
1.2 What are Photonic Processes?
1.3 Fundamental Biology Studies Need Photonics
1.4 Applied Biology — Molecular Medicine Using Photonic Means
1.5 Layout of This Volume
2. Review of Electromagnetic Field Interaction with Matter
2.1 Electromagnetic Field, Intensity, and Photon Numbers
2.2 Classical or Quantum Mechanics Description of Interaction?
2.3 The Radiation Field and Its Quantum Description
2.4 One-photon Matter Interaction
2.4.1 Linear absorption
2.4.2 Birefringence and dichroism
2.4.3 Circular dichroism and rotatory dispersion
2.4.4 Attenuated total reflection (ATR) and totally internal reflection (TIR) spectroscopy
2.4.5 Surface plasmon resonance (SPR)
2.5 Two-photon Matter Interactions
2.5.1 Scattering of light — Structural determination and diffraction
2.5.2 Temporal studies: Dynamic Light Scattering (DLS) and Photon Correlation Spectroscopy (PCS)
2.5.3 Raman scattering (classical perspective)
2.5.4 Fluorescence spectroscopy
2.5.5 Fluorescence Resonant Energy Transfer (FRET) analysis
2.6 Nonlinear Optical Susceptibility
2.6.1 Second-order nonlinearities
2.6.2 Four-wave mixing processes
2.7 Gradient Radiation Pressure ⇒ Optical Trapping
3. Molecular and Cellular Structure
3.1 From Atoms to Molecules
3.1.1 Molecular bonding
3.1.2 Hybridization and the unique carbon bonding capability
3.1.3 Other molecular bonding mechanisms
3.2 Molecules of the Cell
3.2.1 Nucleic acids
3.2.2 Lipids
3.2.3 Proteins
3.2.4 Carbohydrates
4. The Expanse of Biology Problems in Need of Quantitative Solution
4.1 The Need to Measure at Increasingly Higher Spatial Resolution
4.1.1 X-ray diffraction and imaging
4.1.2 Electron microscopy
4.1.3 Nuclear Magnetic Resonance (NMR) spectroscopy
4.1.4 Atomic Force Microscopy (AFM) and Near-field Scanning Optical Microscopy (NSOM)
4.1.5 Optical imaging
4.2 Examples of How Optical Methods Are Used in Relating Structure to Function
4.2.1 Protection, regulation, and specification of proper functions within the cell
4.2.2 Muscle contractility
4.2.3 Genetic management: Replication, search, repair, and damage control
4.2.4 Helicase activity in prokaryotic cells
4.2.5 RNA polymerase-II
4.2.6 Telomere and telomerase
5. Optical Microscopy
5.1 Basics of an Optical Microscope
5.1.1 Summary of the needed parameters for imaging cellular or molecular parameters
5.2 Attempts to Meet the Needs of Higher Clarity in Microscopy
5.2.1 Phase contrast microscopy
5.2.2 Early X-ray microscopy imaging
5.2.3 Confocal microscopy — Beating that Abbe limit #1
5.2.4 Near-field microscopes — Beating Abbe limit #2
5.3 Using Contrast Agents, Intrinsic or Otherwise
5.3.1 Fluorescence or phosphorescence emission enhances contrast
5.3.2 Fluorescent proteins
5.3.3 Quantum dots
5.3.4 Search for better emitters
5.4 Ultrahigh Resolution Fluorescence Microscopy
5.4.1 Stimulated Emission Depletion (STED)
5.4.2 Photo-Activated Localization Microscopy (PALM) and STochastic Optical Reconstruction Microscopy (STORM)
5.4.3 Structured Illumination Microscopy (SIM)
5.5 Multiphoton Excitation Fluorescence
5.6 Summary
6. Label-free High-resolution Microscopy
6.1 Many Faces of Nonlinear Interaction
6.1.1 Second harmonic generation (SHG) and imaging
6.1.2 Sum frequency generation — Possibly spectral imaging
6.1.3 Stimulated Raman Scattering
6.1.4 Coherent Anti-Stokes Raman Scattering (CARS)
6.1.5 Self-focusing and self-phase modulation
6.2 X-ray Microscopy
6.2.1 Early soft X-ray microscopes
6.2.2 Coherent X-ray laser sources for microscopy
7. Temporal Dynamics
7.1 Particle Tracking
7.1.1 Tracking motor molecule dynamics
7.2 Time Correlation Analysis
7.2.1 Photon Correlation Spectroscopy (PCS or Dynamic Light Scattering [DLS])
7.2.2 Fluorescence Correlation Spectroscopy (FCS)
7.3 Progress in the Extension of the FCS Method
7.4 Fluorescence Cross-Correlation Spectroscopy (FCCS)
7.5 Combining Techniques
7.5.1 Multi (or Two) Photon Excited Fluorescence (M(T)PEF) FCS
7.5.2 Evanescent wave-FCS
7.6 Anti-bunching Spectroscopy
7.7 Imaging FCS
7.8 Fluorescence Resonant Energy Transfer (FRET) FCS
7.9 Summary
8. Photonics in Medicine
8.1 Visualization and Validation
8.1.1 Imaging with microscopy and endoscopy
8.1.2 Optical Coherence Tomography (OCT)
8.1.3 Optical endoscope
8.1.4 Adaptive optics microscopy
8.2 Biomarkers
8.2.1 Use of the microRNA detectors
8.2.2 From antibodies to SHALs and aptamers
8.2.3 Multiplexed microbead assays — xMAP technology
8.2.4 DNA microarrays
8.2.5 Nanoparticles
8.2.6 Fluorescence Lifetime Imaging Microscopy (FLIM)
8.3 Photonics Means of Medical Therapy
8.3.1 Photodynamic therapy (PDT)
8.3.2 Mechanism of PDT
8.3.3 Emerging areas of research in PDT
8.4 Controlling Molecular Gating Functions
8.4.1 Mechanism of action
8.5 Final Thoughts
Index
Biophotonics: Science and Technology
This lecture volume aims to give students and researchers in this rapidly expanding field of biophotonics an interdisciplinary perspective. Among the primary topics are ultrahigh resolution microscopy, particle tracking, photon correlation spectroscopy, and nonlinear optical methods as used in biological and biomedical research, with a focus on current applications in biophysics and biomedicine.
9789813235687 (hardcover)
Optical Imaging
Molecular Imaging
Microscopy
Optics and Photonics--methods--methods--methods--methods
616.075 / YEH
WN 195