MARC details
| 000 -LEADER |
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| fixed length control field |
04245cam a2200433Ia 4500 |
| 001 - ACCESSION NUMBER |
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| control field |
10838 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
CUTN |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20131127113002.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS--GENERAL INFORMATION |
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| fixed length control field |
m d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
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| fixed length control field |
cr cn| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
100225s2010 ne a sb 001 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
9780123747273 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
|---|
| International Standard Book Number |
0123747279 |
| 035 ## - SYSTEM CONTROL NUMBER |
|---|
| System control number |
(OCoLC)528609521 |
| 037 ## - SOURCE OF ACQUISITION |
|---|
| Stock number |
166590:166792 |
| Source of stock number/acquisition |
Elsevier Science & Technology |
| Note |
http://www.sciencedirect.com |
| 040 ## - CATALOGING SOURCE |
|---|
| Original cataloging agency |
OPELS |
| Language of cataloging |
eng |
| Transcribing agency |
OPELS |
| 049 ## - LOCAL HOLDINGS (OCLC) |
|---|
| Holding library |
MAIN |
| 050 14 - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
QP623 |
| Item number |
.F37 2010 |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (OCLC) |
|---|
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) |
QP623 |
| Local cutter number (OCLC) ; Book number/undivided call number, CALL (RLIN) |
Far 2010 |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Farrell, Robert E. |
| Locally assigned cutter number (author) |
FAR |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
RNA methodologies |
| Medium |
[electronic resource] : |
| Remainder of title |
laboratory guide for isolation and characterization / |
| Statement of responsibility, etc |
Robert E. Farrell Jr. |
| 250 ## - EDITION STATEMENT |
|---|
| Edition statement |
4th ed. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc |
Amsterdam ; |
| -- |
Boston : |
| Name of publisher, distributor, etc |
Elsevier/Academic Press, |
| Date of publication, distribution, etc |
2010. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xxiii, 717 p. : |
| Other physical details |
ill. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Previous ed.: 2005. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc |
Includes bibliographical references and index. |
| 505 0# - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
CHAPTER 1: RNA and the Cellular Biochemistry Revisited -- CHAPTER 2: RNA Isolation Strategies -- CHAPTER 3: The Truth about Tissues -- CHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW) -- CHAPTER 5: Isolation of Polyadenylated RNA -- CHAPTER 6: Quality Control for RNA Preparations (EXPANDED) -- CHAPTER 7: Resilient Ribonucleases -- CHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED) -- CHAPTER 9: Electrophoresis of RNA (EXPANDED) -- CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED) -- CHAPTER 11: Northern Analysis -- CHAPTER 12: Nucleic Acid Probe Technology -- CHAPTER 13: Practical Nucleic Acid Hybridization -- CHAPTER 14: Principles of Detection (EXPANDED) -- CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection -- CHAPTER 16: Analysis of Nuclear RNA (EXPANDED) -- CHAPTER 17: cDNA Synthesis (EXPANDED) -- CHAPTER 18: RT-PCR (NEW) -- CHAPTER 19: Quantitative PCR Techniques -- CHAPTER 20: Functional Genomics and Transcript Profiling (NEW) -- CHAPTER 21: High-Throughput Analysis of Gene Expression -- CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW) -- CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED) -- CHAPTER 24: Genomes, Transcriptomes, Proteomes, and Bioinformatics -- CHAPTER 25: An RNA Paradigm. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc |
This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. |
| 533 ## - REPRODUCTION NOTE |
|---|
| Type of reproduction |
Electronic reproduction. |
| Place of reproduction |
Amsterdam : |
| Agency responsible for reproduction |
Elsevier Science & Technology, |
| Date of reproduction |
2010. |
| Note about reproduction |
Mode of access: World Wide Web. |
| -- |
System requirements: Web browser. |
| -- |
Title from title screen (viewed on Feb. 24, 2010). |
| -- |
Online version restricted to NUS staff and students only through NUSNET. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
RNA |
| General subdivision |
isolation & purification |
| Form subdivision |
Laboratory Manuals. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Molecular Biology |
| General subdivision |
methods |
| Form subdivision |
Laboratory Manuals. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
RNA |
| General subdivision |
physiology |
| Form subdivision |
Laboratory Manuals. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
RNA |
| General subdivision |
Analysis |
| Form subdivision |
Laboratory manuals. |
| 655 #7 - INDEX TERM--GENRE/FORM |
|---|
| Genre/form data or focus term |
Electronic books. |
| Source of term |
local. |
| 710 2# - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
ScienceDirect (Online service) |
| 776 1# - ADDITIONAL PHYSICAL FORM ENTRY |
|---|
| Qualifying information |
Original |
| International Standard Book Number |
9780123747273 |
| -- |
0123747279 |
| Record control number |
(DLC) 2005041012 |
| -- |
(OCoLC)496226598. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
General Books |
| Koha issues (borrowed), all copies |
1 |
| 956 40 - LOCAL ELECTRONIC LOCATION AND ACCESS (OCLC) |
|---|
| Uniform Resource Identifier |
<a href="http://libproxy1.nus.edu.sg/login?url=http://www.sciencedirect.com/science/book/9780123747273">http://libproxy1.nus.edu.sg/login?url=http://www.sciencedirect.com/science/book/9780123747273</a> |
| Public note |
View the electronic version (full-text) via sciencedirect |
