Protein chromatography : methods and protocols / edited by Dermot Walls, School of Biotechnology, Dublin City University, Dublin, Ireland, Sinéad T. Loughran, Department of Applied Sciences, Dundalk Institute of Technology, Dublin, Ireland.
Material type: TextLanguage: English Series: Methods in molecular biology ; 1485Publication details: New York, N.Y. : Humana Press, ©2017.Edition: Second editionDescription: x, 426 pages : illustrations (some color) ; 27 cmISBN:- 9781493964109 (hardback)
- 572.6 23 WAL
Item type | Current library | Collection | Call number | Status | Date due | Barcode |
---|---|---|---|---|---|---|
Reference Books | CUTN Central Library Sciences | Non-fiction | 572.6 WAL (Browse shelf(Opens below)) | Not For Loan | 41797 |
Browsing CUTN Central Library shelves, Shelving location: Sciences, Collection: Non-fiction Close shelf browser (Hides shelf browser)
No cover image available No cover image available | ||||||||
572.6 DAL Protein targeting, transport & translocation | 572.6 HEP Basic Bioinformatics / | 572.6 OME Exploring the human plasma proteome / | 572.6 WAL Protein chromatography : | 572.65 DEL Amino Acids And Proteins | 572.7 SHU Chemistry of enzymes | 572.76 JAN RNA helicases / |
"Springer Protocols"--Cover.
Part I. Overview Chapters Part II. Protocols Chapters
This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.
Includes bibliographical references and index.
There are no comments on this title.