Atomic force microscopy : biomedical methods and applications / edited by Pier Carlo Braga, Davide Ricci.Material type: TextLanguage: English Series: Methods in molecular biology (Clifton, N.J.) ; v. 242.Publication details: Totowa, N.J. : Humana Press, ©2004. Description: 1 online resource (xiv, 394 pages) : illustrationsISBN: 1588290948; 9781588290946; 1592596479; 9781592596478; 128084311X; 9781280843112; 9786610843114; 6610843112Subject(s): Atomic force microscopy | Microscopy, Atomic Force | Molecular Biology | NATURE | SCIENCE | SCIENCE | Atomic force microscopy | -- methods | -- methods | -- Reference | -- Life Sciences -- General | -- Life Sciences -- Biology | Additional physical formats: Print version:: Atomic force microscopy.DDC classification: 570.282 NLM classification: W1 | QH 212.A78Online resources: Click here to access online | SpringerLink eBooks
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Analysis of human fibroblasts by atomic force microscopy / Gillian R. Bushell [and others] -- Corneal tissue observed by atomic force microscopy Stylliani Lydataki [and others] -- AFM study of surface structure changes in mouse spermatozoa associated with maturation / Hiroko Takano and Kazuhiro Abe -- Calculation of cuticle step heights from AFM images of outer surfaces of human hair / James R. Smith -- Imaging living chondrocyte surface structures with AFM contact mode / Gerlinde Bischoff [and others] -- Growth cones of living neurons probed by atomic force microscopy / Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco -- Evaluating demineralization and mechanical properties of human dentin with AFM / Grayson W. Marshall Jr. [and others] -- Applying atomic force microscopy to studies in cardiac physiology / Jason J. Davis, Trevor Powell, and H. Allen O. Hill -- Imaging bacterial shape, surface, and appendages before and after treatments with antibiotics / Pier Carlo Braga and Davide Ricci.
How the Atomic Force microscope works / Davide Ricci and Pier Carlo Braga -- Imaging methods in atomic force microscopy / Davide Ricci and Pier Carlo Braga -- Recognizing and avoiding artifacts in AFM imaging / Davide Ricci and Pier Carlo Braga -- Advanced biosensing using micromechanical cantilever arrays / Martin Hegner and Youri Arntz.
Measurement of mechanical properties of intact endothelial cells in fresh arteries / Hiroshi Miyazaki and Kozaburo Hayashi -- Observation of oxidative stress on yeast cells / Ricardo de Souza Pereira -- Lymphoblastoid cells exposed to low-frequency magnetic fields: study by atomic force microscopy / Settimio Grimaldi, Marco Girasole, and Antonio Cricenti -- Sample preparation method for observing RNA polymerase activity by atomic force microscopy / Sandor Kasas -- Atomic force microscopy of B-Amyloid: static and dynamic studies of nanostructure and its formation / Justin Legleiter and Tomasz Kowalewski -- How to build up biosensors with the cantilever of the atomic force microscope / Ricardo de Souza Pereira -- Measurement of single molecular interactions by dynamic force microscopy / Martin Hegner, Wilfried Grange, and Patricia Bertoncini.
Visualizing nuclear structure In Situ by atomic force microscopy / Luis Felipe Jimnez-Garcia and Mar a de Lourdes Segura-Valdez -- Imaging surface and submembranous structures in living cells with the atomic force microscope: notes and tricks / Filip Braet and Eddie Wisse -- Atomic force microscopy of protein complexes / Olga I. Kiselyova and Igor V. Yaminsky -- Atomic force microscopy of interfacial monomolecular films of pulmonary surfactant / Kaushik Nag [and others] -- High-resolution analysis of the 3D organization of human metaphase chromosomes / Stefan Thalhammer [and others] -- Shape and volume of living aldosterone-sensitive cells imaged with the atomic force microscope / Stefan W. Schneider [and others] -- Localization of epithelial sodium channels by atomic force microscopy / Peter R. Smith and Dale J. Benos -- High-resolution AFM imaging of bacteriorhodopsin / Dimitrios Fotiadis and Andreas Engel.
Although atomic force microscopy (AFM) offers many significant advantages over the conventional microscopies used in the biological and medical sciences, its use is more familiar to physicists and engineers than to biomedical researchers. In Atomic Force Microscopy: Biomedical Methods and Applications, highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. Each tested protocol includes step-by-step instructions to ensure successful experimental results, background material on the principle behind the technique, tips on troubleshooting and avoiding known pitfalls, and notes on how to distinguish artifacts from useful data. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection. Cutting-edge and highly practical, Atomic Force Microscopy: Biomedical Methods and Applications will help all investigators in biology and medicine open a new microscopic world, develop novel applications, and apply this powerful technology productively in their own work.
Includes bibliographical references and index.