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The condensed protocols from Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.

By: Contributor(s): Material type: TextTextPublication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006.Description: v, 800 p. : ill. ; 28 cmISBN:
  • 9780879698126 (hardcover : alk. paper)
  • 0879697717 (pbk. : alk. paper)
  • 9780879697716
Subject(s): DDC classification:
  • 572.8 22
LOC classification:
  • QH442.2 .S24 2006
Online resources:
Contents:
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
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Holdings
Item type Current library Call number Copy number Status Date due Barcode
General Books General Books CUTN Central Library Sciences 572.8 (Browse shelf(Opens below)) 1 Available 12694
General Books General Books CUTN Central Library Sciences 572.8 (Browse shelf(Opens below)) 2 Available 19756
General Books General Books CUTN Central Library Sciences 572.8 (Browse shelf(Opens below)) 3 Available 19757

"A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--P. [4] of cover.

Includes bibliographical references and index.

Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.

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