The condensed protocols from Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell.
Material type: TextPublication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2006.Description: v, 800 p. : ill. ; 28 cmISBN:- 9780879698126 (hardcover : alk. paper)
- 0879697717 (pbk. : alk. paper)
- 9780879697716
- 572.8 22
- QH442.2 .S24 2006
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---|---|---|---|---|---|---|
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General Books | CUTN Central Library Sciences | 572.8 (Browse shelf(Opens below)) | 2 | Available | 19756 | |
General Books | CUTN Central Library Sciences | 572.8 (Browse shelf(Opens below)) | 3 | Available | 19757 |
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"A single-volume adaptation of the three-volume third edition of Molecular cloning: a laboratory manual"--P. [4] of cover.
Includes bibliographical references and index.
Plasmids and their usefulness in molecular cloning -- Bacteriophage and its vectors -- Working with bacteriophage M13 vectors -- Working with high-capacity vectors -- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis -- Preparation and analysis of eukaryotic genomic DNA -- Extraction, purification, and analysis of mRNA from eukaryotic cells -- In vitro amplification of DNA by the polymerase chain reaction -- Preparation of radiolabeled DNA and RNA probes -- Working with synthetic oligonucleotide probes -- Preparation of cDNA libraries and gene identification -- DNA sequencing -- Mutagenesis -- Screening expression libraries -- Expression of cloned genes in escherichia coli -- Introducing cloned genes into cultured mammalian cells -- Analysis of gene expression in cultured mammalian cells -- Protein interaction technologies.
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