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Experimental Biotechnology : A Laboratory Manual / Sunita Dutta, Abhijit Dutta and Ashok Kumar Choudhary

By: Contributor(s): Material type: TextTextLanguage: English Series: Practical Manual Series - ; 6Publication details: New Delhi : New India Publishing Agency, 2011.Description: xii, 254 p. : ill. ; 22.86 cmISBN:
  • 9789380235721
Subject(s): DDC classification:
  • 22 660.6 DUT
Contents:
01. General procedures. 02. Nucleic acids and E.coli. 03. Enzymes. 04. Cloning. 05. DNA/RNA separation. 06. Proteins. 07. Plant biotechnology.
Summary: The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.
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Item type Current library Collection Call number Status Date due Barcode
General Books General Books CUTN Central Library Sciences Non-fiction 660.6 DUT (Browse shelf(Opens below)) Available 40552

01. General procedures. 02. Nucleic acids and E.coli. 03. Enzymes. 04. Cloning. 05. DNA/RNA separation. 06. Proteins. 07. Plant biotechnology.

The book is subdivided into seven sections this encompass: general procedures, like methods of pipetting, solution preparation, buffers and principles of common analytical instruments essential for laboratory biotechnology experiments. The book also includes working with nucleic acid, bacteria, enzymes, proteins; cloning experiments and a few protocols on plant biotechnology. Emphasis have been given on DNA/RNA isolation from various sources, use of restriction enzymes, ligation techniques, cloning protocols, screening of transformed cells, various electrophoresis techniques, PCR protocol, etc. The appendices in the last part are included to provide information important to he study of the above-mentioned practical as a whole. The book will be useful to students belonging to Biotechnology, agriculture and allied fields. The idea behind this practical manual was thus to provide theoretical basis of the practical study items to be undertaken in the laboratory in a lucid manner.

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