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Antibodies : a laboratory manual / edited by Edward A. Greenfield, Dana-Farber Cancer Institute.

Contributor(s): Material type: TextTextEdition: Second editionDescription: xxi, 847 pages : illustrations ; 29 cmISBN:
  • 9781936113804 (hardback)
  • 1936113805 (hardback)
  • 9781936113811 (paper)
  • 1936113813 (paper)
Uniform titles:
  • Using antibodies.
Subject(s): DDC classification:
  • 571.9/67 23
LOC classification:
  • QR186.7 .H37 2014
Other classification:
  • SCI010000 | SCI007000 | SCI049000
Contents:
Machine generated contents note: -- Preface -- 1 Antibody Production by the Immune System -- Stefanie Sarantopoulos -- 2 The Antibody Molecule -- Stefanie Sarantopoulos -- 3 Antibody-Antigen Interactions -- Stefanie Sarantopoulos -- 4 Antibody Responses -- Stefanie Sarantopoulos -- 5 Selecting the Antigen -- Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam -- 6 Immunizing Animals -- Edward A. Greenfield -- 7 Generating Monoclonal Antibodies -- Edward A. Greenfield -- 8 Growing Hybridomas -- Edward A. Greenfield -- 9 Characterizing Antibodies -- Frances Weis-Garcia and Robert H. Carnahan -- 10 Antibody Purification and Storage -- Jordan B. Fishman and Eric A. Berg -- 11 Engineering Antibodies -- James Dasch and Amy Dasch -- 12 Labeling Antibodies -- Eric A. Berg and Jordan B. Fishman -- 13 Immunoblotting -- Larisa Litovchick -- 14 Immunoprecipiation -- James DeCaprio and Thomas O. Kohl -- 15 Immunoassays -- Thomas O. Kohl and Carl A. Ascoli -- 16 Cell Staining -- Scott J. Rodig -- 17 Antibody Screening using High Throughput Flow Cytometry -- Thomas D.l. Duensing and Susan R. Watson -- Appendix I: Electrophoresis -- Appendix II: Protein Techniques -- Appendix III: General Information -- Appendix IV: Bacterial Expression -- Appendix V: Cautions -- Index.
Summary: "The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"--Other editions: Revision of:: Harlow, Edward. Using antibodies
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Holdings
Item type Current library Call number Status Date due Barcode
General Books General Books CUTN Central Library Sciences 571.9/67 (Browse shelf(Opens below)) Available 19990

Includes bibliographical references and index.

Machine generated contents note: -- Preface -- 1 Antibody Production by the Immune System -- Stefanie Sarantopoulos -- 2 The Antibody Molecule -- Stefanie Sarantopoulos -- 3 Antibody-Antigen Interactions -- Stefanie Sarantopoulos -- 4 Antibody Responses -- Stefanie Sarantopoulos -- 5 Selecting the Antigen -- Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam -- 6 Immunizing Animals -- Edward A. Greenfield -- 7 Generating Monoclonal Antibodies -- Edward A. Greenfield -- 8 Growing Hybridomas -- Edward A. Greenfield -- 9 Characterizing Antibodies -- Frances Weis-Garcia and Robert H. Carnahan -- 10 Antibody Purification and Storage -- Jordan B. Fishman and Eric A. Berg -- 11 Engineering Antibodies -- James Dasch and Amy Dasch -- 12 Labeling Antibodies -- Eric A. Berg and Jordan B. Fishman -- 13 Immunoblotting -- Larisa Litovchick -- 14 Immunoprecipiation -- James DeCaprio and Thomas O. Kohl -- 15 Immunoassays -- Thomas O. Kohl and Carl A. Ascoli -- 16 Cell Staining -- Scott J. Rodig -- 17 Antibody Screening using High Throughput Flow Cytometry -- Thomas D.l. Duensing and Susan R. Watson -- Appendix I: Electrophoresis -- Appendix II: Protein Techniques -- Appendix III: General Information -- Appendix IV: Bacterial Expression -- Appendix V: Cautions -- Index.

"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"--

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