Identification and Characterization of Plant Circular RNAs -- Experimental Strategies for Studying the Function of Plant circRNAs -- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization -- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development -- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing -- Identification and Functional Characterization of Viroid Circular RNAs -- Circular RNA Databases -- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs -- Computational Analysis of Transposable Elements and circRNAs in Plants -- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database -- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.

This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs' research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field. .