Plant Biochemistry/ G.Nagaraj

art I. Analytical Techniques

1. Introduction
2. Chromatography
3. Paper Chromatography
4. Column Chromatography
5. Thin Layer Chromatography
6. Electrophoresis
7. Ion Exchange Chromatography
8. Gel-filtration Chromatography
9. Solvent Extraction
10. Dialysis, Ultra Filtration and Lyophilisation
11. Centrifugation
12. Gas Liquid Chromatography
13. High Pressure Liquid Chromatography
14. GC/MS and LC/MS
15. Spectrophotometry
16. Infra Red Spectroscopy
17. Flame Photometry
18. Atomic-absorption Spectroscopy
19. Inductively Coupled Plasma Atomic Emission Spectroscopy
20. NMR Spectroscopy
21. Radiochemical Methods
22. Mass Spectrometry
23. Polarimetry
24. Conductimetry
25. Potentiometry
26. ELISA and Radioimmunoassay (RIA)
27. Gravimetric Analysis
28. Volumetric Analysis
29. Amplification and Sequencing of Nucleic Acids

Part II. Analytical Methods and Procedures

1. Methods of Analysis
2. Carbohydrates and their Methods of Analysis
3. Fats/Oils/Lipophilic Constituents
4. Amino Acids and Proteins
5. Enzymes
6. Nucleic Acids
7. Vitamines
8. Phenolics and Antinutrients
9. Toxins
10. Alkaloids
11. Plant Nutrients
12. Plant Pigments
13. Pesticide Residue Analysis

This book is aimed at helping the analyst with respect to analysis of samples, mainly, plant material. The book is divided into two parts. o The first part deals with the different techniques involved in the biochemical analysis. The theoretical aspects of the same are presented in detail. The mechanism and some information on the buildup of the equipments are furnished. The need and scope of the instruments and their utility are also presented. o The second part deals with specific methods of analysis. Analytical methods of sugars, amino acids, proteins, fats, enzymes and their components are presented. Nucleic acid, vitamin, antinutrient (toxicants) and mineral analytical methods also have been included. o Information on alkaloid and pesticide residue analytical methods etc. has also been furnished. In many cases two or more methods have been given. o They have been given in such a way that they include reagents, apparatus and step wise procedures. o Final calculations needed for arriving at the concentration of the constituents have also been included.