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Calcium Techniques : A Laboratory Manual / edited by Jan B. Parys, KU Leuven, Martin D. Bootman, The Open University, David I. Yule, University of Rochester, Geert Bultynck, KU Leuven.

Contributor(s): Material type: TextTextPublication details: New York ; Cold Spring Harbor Laboratory Press, c2014.Description: xvi, 592 pages : illustrations (some color) ; 28 cmISBN:
  • 9781621820789 (hbk.)
  • 1621820785 (hbk.)
  • 9781936113583 (pbk.)
  • 1936113589 (pbk.)
Subject(s): DDC classification:
  • 572/.516 23
LOC classification:
  • QP535.C2 C35 2014
NLM classification:
  • QV 25
Contents:
Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.
Summary: "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
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Holdings
Item type Current library Call number Status Barcode
General Books General Books CUTN Central Library Sciences 572/.516 (Browse shelf(Opens below)) Available 19780

Includes bibliographical references and index.

Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.

"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--

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