MicroRNA protocols / edited by Shao-Yao Ying.

By:

Ying, Shao-Yao

Contributor(s):

Ying, Shao-Yao

Material type: Text

TextLanguage: English Series: Methods in molecular biology (Clifton, N.J.) ; v. 342.Publication details: Totowa, N.J. : Humana Press, c2006.Description: xiii, 365 p. : ill. (some col.) ; 24 cmISBN:

1588295818 (alk. paper)
1597451231 (eISBN)
9781588295811

Subject(s):

DDC classification:

572.88 22 YAO

NLM classification:

W1
QU 58.7

Online resources:

Contents:

The microRNA: overview of the RNA gene that modulates gene functions Structure analysis of microRNA precursors MicroRNA biogenesis: isolation and characterization of the microprocessor complex Recognition and cleavange of primary microRNA transcripts Mouse embryonic stem cells as a model genetic system to dissect and exploit the RNA interference machinery MicroRNAs and messenger RNA turnover Prediction of microRNA targets Prediction of human microRNA targets Complications in mammalian microRNA target prediction miRBase: the microRNA sequence database In situ hybridization as a tool to study the role of microRNAs in plant development Usefulness of the luciferase reporter system to test in efficacy of siRNA Cloning microRNAs from mammalian tissues Methods for analyzing microRNA expression and function during hematopoietic lineage differentiation Identifying microRNNA regulators of cell death in Drosophila MicroRNAs in human immunodeficiency virus-1 infection Cloning and detection of HIV-1-encoded microRNA microRNAs associated with fragile X mental retardation protein In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector Gene silencing in vitro and in vivo using intronic microRNAs Transgene-link animal models using intronic microRNAs Evolution of microRNAs Perspectives

Summary: It is now known that microRNA (miRNA), once thought to be junk, can suppress the expression of other genes and may be involved in numerous cellular processes, including cell proliferation, apoptosis, cell differentiation, and development. In MicroRNA Protocols, expert miRNA researchers explain its basic concepts and introduce the most advanced technologies and techniques for predicting screening, isolating, and Read more...

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Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
General Books	CUTN Central Library Sciences	Non-fiction	572.88 YAO (Browse shelf(Opens below))	Available		38288

The microRNA: overview of the RNA gene that modulates gene functions
Structure analysis of microRNA precursors
MicroRNA biogenesis: isolation and characterization of the microprocessor complex Recognition and cleavange of primary microRNA transcripts
Mouse embryonic stem cells as a model genetic system to dissect and exploit the RNA interference machinery MicroRNAs and messenger RNA turnover
Prediction of microRNA targets
Prediction of human microRNA targets
Complications in mammalian microRNA target prediction miRBase: the microRNA sequence database In situ hybridization as a tool to study the role of microRNAs in plant development
Usefulness of the luciferase reporter system to test in efficacy of siRNA Cloning microRNAs from mammalian tissues Methods for analyzing microRNA expression and function during hematopoietic lineage differentiation Identifying microRNNA regulators of cell death in Drosophila
MicroRNAs in human immunodeficiency virus-1 infection Cloning and detection of HIV-1-encoded microRNA microRNAs associated with fragile X mental retardation protein In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector Gene silencing in vitro and in vivo using intronic microRNAs Transgene-link animal models using intronic microRNAs
Evolution of microRNAs
Perspectives

It is now known that microRNA (miRNA), once thought to be junk, can suppress the expression of other genes and may be involved in numerous cellular processes, including cell proliferation, apoptosis, cell differentiation, and development. In MicroRNA Protocols, expert miRNA researchers explain its basic concepts and introduce the most advanced technologies and techniques for predicting screening, isolating, and Read more...

Includes bibliographical references and index.

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