Microbial carotenoids from bacteria and microalgae : methods and protocols / edited by Jose-Luis Barredo.

Pathways of carotenoid biosynthesis in bacteria and microalgae / Selection and taxonomic identification of carotenoid-producing marine actinomycetes / Isolation, characterization, and diversity of novel radiotolerant carotenoid-producing bacteria / Novel radio-tolerant astaxanthin-producing bacterium reveals a new astaxanthin derivative : astaxanthin dirhamnoside / Novel zeaxanthin-producing bacteria isolated from a radioactive hot spring water / Novel approach in the biosynthesis of functional carotenoids in Escherichia coli / Engineering Escherichia coli for canthaxanthin and ataxanthin biosynthesis / Analysis of canthaxanthin production by Gordonia jacobaea / Isolation and light-stimulated expression of canthaxanthin and spirilloxanthin biosynthesis genes from the photosynthetic bacterium Bradyrhizobium sp. Strain ORS278 / Construction of carotenoid biosynthetic pathways through chromosomal integration in methane-utilizing bacterium Methylomonas sp. strain 16a / Genetic modification in Bacillus subtilis for production of C30 carotenoids / Carotenoids' production from halophilic bacteria / Construction and utilization of carotenoid reporter systems : identification of chromosomal integration sites that support suitable expression of biosynthetic genes and pathways / Directed evolution of carotenoid synthases for the production of unnatural carotenoids / High-Throughput Screen for the Identi fi cation of improved catalytic activity: b-carotene hydroxylase / DNA fingerprinting intron-sizing method to accomplish a specific, rapid, and sensitive identification of carotenogenic Dunaliella species / Ketocarotenoid biosynthesis in transgenic microalgae expressing a foreign beta-C-4-carotene oxygenase gene / Characterization of carotenogenesis genes in the cyanobacterium Anabaena sp. PCC 7120 / Obtaining lutein-rich extract from microalgal biomass at preparative scale / NMR-based isotopologue profiling of microbial carotenoids / Analysis of diapocarotenoids found in pigmented Bacillus species /

Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage and may be responsible for the colours associated with plants and animals. In Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols, expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the study of canthaxanthin production, construction of carotenoid reporter systems, directed evolution of carotenoid synthases, and improvement of b-carotene hydroxylase catalytic activity are described. Additionally, the book includes methods of DNA fingerprinting for the identification of carotenogenic Dunaliella species, ketocarotenoid biosynthesis in microalgae expressing the beta-C-4-carotene oxygenase gene, characterization of carotenogenesis genes in Anabaena sp., obtaining lutein from microalgal biomass, NMR-based isotopologue profiling of microbial carotenoids, and analysis of diapocarotenoids. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms.

Includes bibliographical references and index.

J. Paniagua-Michel, Jorge Olmos-Soto, and Manuel Acosta Ruiz -- Francisco Romero -- Dalal Asker [and others] -- Dalal Asker [and others] -- Dalal Asker [and others] -- Hisashi Harada and Norihiko Misawa -- Qiong Cheng and Luan Tao -- Patricia Veiga-Crespo [and others] -- Eric Giraud and Andre Vermeglio -- Rick W. Ye and Kristen Kelly -- Isamu Maeda -- Maria de Lourdes Moreno [and others] -- Pamela L. Sharpe and Deana J. DiCosimo -- Maiko Furubayashi and Daisuke Umeno -- Mark A. Scaife [and others] -- Jorge Olmos-Soto [and others] -- Marta Vila [and others] -- Hajime Masukawa, Mari Mochimaru, and Shinichi Takaichi -- Jose M. Fernandez-Sevilla, F. Gabriel Acien Fernandez, and Emilio Molina Grima -- Eva Eylert, Adelbert Bacher, and Wolfgang Eisenreich -- Laura Perez-Fons and Paul D. Fraser.

English.