Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean / Phetole Mangena.

By:

Mangena, Phetole

Material type: Text

TextLanguage: English Publication details: Florida : CRC Press, 2023.Edition: 1st edDescription: xii, 226p. : Col ill. ; 22 cmISBN:

9781032250380

Uniform titles:

Plant Transformation via Agrobacterium Tumefaciens: Culture Conditions, Recalcitrance and Advances in Soybean

Subject(s):

DDC classification:

23 577.57 MAN

Contents:

Overview of Genetic Transformation in Plants Plant Transformation History Agrobacterium Tumefaciens Getting Started with In Vitro Soybean Transformation In Vitro Cultures Commonly used for Plant Transformation Current and Longstanding Challenges facing Soybean Transformation Alternative Techniques for Genetic Manipulations via A. Tumefaciens Molecular Aspects of Indirect Gene Transfer via A. Tumefaciens Genetic Transformation in Other Leguminous Crops Transgenic Plants for Biotic Stress Resistance Transgenic Plants for Abiotic Stress Resistance Potential Health Safety Concerns and Environmental Risks The Regulatory Management of Transgenic Plants Current and Future Prospects in Genetic Engineering Photographic Index of Soybean Transformation Cultures

Summary: Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes. This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment. Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

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Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Text Books	CUTN Central Library Sciences	Non-fiction	577.57 MAN (Browse shelf(Opens below))	Available		47701

Overview of Genetic Transformation in Plants

Plant Transformation History

Agrobacterium Tumefaciens

Getting Started with In Vitro Soybean Transformation

In Vitro Cultures Commonly used for Plant Transformation

Current and Longstanding Challenges facing Soybean Transformation

Alternative Techniques for Genetic Manipulations via A. Tumefaciens

Molecular Aspects of Indirect Gene Transfer via A. Tumefaciens

Genetic Transformation in Other Leguminous Crops

Transgenic Plants for Biotic Stress Resistance

Transgenic Plants for Abiotic Stress Resistance

Potential Health Safety Concerns and Environmental Risks

The Regulatory Management of Transgenic Plants

Current and Future Prospects in Genetic Engineering

Photographic Index of Soybean Transformation Cultures

Plant Transformation via Agrobacterium Tumefaciens compiles fundamental and specific information and procedures involving in vitro soybean transformation, which forms the basis for the Agrobacterium-mediated genetic manipulation of soybean using plant tissue culture. This method serves as one of the most preferred, reliable and cost-effective mechanism of transgene expression in both leguminous recalcitrant species and non-legume crops. The technology is favoured due to its simplicity, feasibility and high transformation rates that are so far achieved mostly in monocot plants and a few dicot genotypes.

This book provides a comprehensive review of plant transformation which remains necessary for many researchers who are still facing protocol-related hurdles. Among some of the major topics covered in Plant Transformation via Agrobacterium Tumefaciens are the history and discovery of Agrobacterium bacterium, longstanding challenges causing transformation inefficiencies, types and conditions of explants, development of transgenic plants for stress resistance, and the role of transgenic plants on animal/human health, including the environment.

Plant Transformation via Agrobacterium Tumefaciens helps the reader to understand how soybean, like many other orphan legume crops, faces the risk of overexploitation which may render the currently available varieties redundant and extinct should its narrow gene pool not improve. Plant transformation serves as a key technique in improving the gene pool, while developing varieties that are drought tolerant, have enhanced nutritional value, pest resistant and reduce the destruction by disease causing microorganims. This book is an essential foundation tool that is available for researchers and students to reinforce the application of Agrobacterium-mediated genetic transformation in soybean.

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