Introduction to instrumentation in life sciences / Prakash S. Bisen, Anjana Sharma.

By:

Bisen, Prakash S

Contributor(s):

Sharma, Anjana (Of Rani Durgavati Vishwavidyalaya)

Material type: Text

TextPublication details: Boca Raton : CRC Press, 2013.Description: xix, 355 p. : ill. (some col.) ; 26 cmISBN:

9781466512405 (pbk. : alk. paper)

Subject(s):

DDC classification:

570.284 23 BIS

LOC classification:

QH324.42 .B57 2013

NLM classification:

QH 324.42

Contents:

Microscopy Introduction Magnification, Resolution, and Contrast Light (Bright Field) Microscopy Dark Field Microscope Phase Contrast Microscope Interference Microscope UV and Fluorescence Microscopes Electron Microscopy Tunneling Electron Microscopy Confocal Microscopy Techniques in Microscopy Electron Microscopy Suggested Readings Important Links Micrometry Introduction Structure of an Ocular Micrometer Conjugate Image-Forming Focal Planes Eyepiece Designs Stage Micrometer Filar Eyepiece Micrometer Principle of Filar Eyepiece Micrometer Working Suggested Reading Important Links Electrochemical Techniques Introduction Structure of a pH Meter Principles Factors Limiting the Accuracy of pH Measurements Measurement of pH Working Procedure for Measuring pH Using a pH Meter Cleaning and Troubleshooting of Glass Probes Application of pH Measurements Oxygen Electrode Suggested Readings Important Links Chromatography Introduction General Principles Column Chromatography Paper Chromatography and Thin-Layer Chromatography Sample Collection, Preservation, and Preparation Adsorption Chromatography Partition Chromatography High-Performance Liquid Chromatography Fast Protein Liquid Chromatography Gas–Liquid Chromatography Ion-Exchange Chromatography Exclusion Chromatography Affinity Chromatography Suggested Readings Important Links Spectroscopy Introduction UV–Visible Spectroscopy IR (Vibrational) Spectroscopy Flame/Atomic Absorption Spectroscopy Fluorescence Spectroscopy ESR Spectroscopy NMR Spectroscopy MALDI-TOF Mass Spectrometry Circular Dichroism (CD) Spectroscopy Suggested Readings Important Links Centrifugation Introduction Principle of Centrifugation Types of Centrifuges Types of Centrifugal Separations Rotor Categories Selection of Centrifuge Tubes Common Centrifugation Vocabulary and Formulas Analytical Ultracentrifuge Suggested Reading Important Links Electrophoresis Introduction Principles of GE Working with the Electrophoresis Apparatus Denaturing Gradient GE Temperature Gradient GE Pulsed-Field GE Capillary Electrophoresis Suggested Readings Important Links X-Ray Microanalysis Introduction Principles Instrumentation Applications of X-Ray Microanalysis Techniques for the Analysis of Secondary, Tertiary, and Quaternary Proteins by X-Ray Crystallography Suggested Readings Important Link Techniques with Radioisotopes Introduction Isotopes and Radioactivity Ionization Effects Measurement Units Measurement Techniques Autoradiography Counting Statistics Biological Uses of Radioisotopes Tracer Dilution Technique Radioimmunoassay Miscellaneous Uses of Radioisotopes Radioisotope Production Most Widely Used Radioisotopes Suggested Reading Important Links Fermentation Introduction History Principles of Fermentation Introduction to Microorganisms Types of Fermentation Metabolism Fermentation Process Types of Fermentation Types of Fermentors Sterilization in Fermentation Product Recovery Suggested Readings Important Links Conductivity Meter Introduction Principles Common Definitions Conductivity Meter Conductivity Cells Factors Influencing the Measurement Measuring Techniques Reliable Measurements Maintenance and Storage Applications Suggested Readings Important Links Glossary Index

Summary: "Designed to serve a multitude section of students and researchers diversified in the field of Life Sciences, Pharmacy, Biotechnology, Microbiology, Biochemistry, and Environmental Sciences, this book addresses the different aspects of instrumentation that hold the key for cutting edge research and innovative application. The authors emphasize techniques encountered both in the practical classes and also in high through put techniques involved in modern era of commercialization and product inventories. It includes illustrated diagrams to explain the principles and theories behind these instruments"--Provided by publisher.

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Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
General Books	CUTN Central Library Sciences	Non-fiction	570.284 BIS (Browse shelf(Opens below))	Available		47292

Includes bibliographical references and index.

Microscopy
Introduction
Magnification, Resolution, and Contrast
Light (Bright Field) Microscopy
Dark Field Microscope
Phase Contrast Microscope
Interference Microscope
UV and Fluorescence Microscopes
Electron Microscopy
Tunneling Electron Microscopy
Confocal Microscopy
Techniques in Microscopy
Electron Microscopy
Suggested Readings
Important Links

Micrometry
Introduction
Structure of an Ocular Micrometer
Conjugate Image-Forming Focal Planes
Eyepiece Designs
Stage Micrometer
Filar Eyepiece Micrometer
Principle of Filar Eyepiece Micrometer
Working
Suggested Reading
Important Links

Electrochemical Techniques
Introduction
Structure of a pH Meter
Principles
Factors Limiting the Accuracy of pH Measurements
Measurement of pH
Working
Procedure for Measuring pH Using a pH Meter
Cleaning and Troubleshooting of Glass Probes
Application of pH Measurements
Oxygen Electrode
Suggested Readings
Important Links

Chromatography
Introduction
General Principles
Column Chromatography
Paper Chromatography and Thin-Layer Chromatography
Sample Collection, Preservation, and Preparation
Adsorption Chromatography
Partition Chromatography
High-Performance Liquid Chromatography
Fast Protein Liquid Chromatography
Gas–Liquid Chromatography
Ion-Exchange Chromatography
Exclusion Chromatography
Affinity Chromatography
Suggested Readings
Important Links

Spectroscopy
Introduction
UV–Visible Spectroscopy
IR (Vibrational) Spectroscopy
Flame/Atomic Absorption Spectroscopy
Fluorescence Spectroscopy
ESR Spectroscopy
NMR Spectroscopy
MALDI-TOF Mass Spectrometry
Circular Dichroism (CD) Spectroscopy
Suggested Readings
Important Links

Centrifugation
Introduction
Principle of Centrifugation
Types of Centrifuges
Types of Centrifugal Separations
Rotor Categories
Selection of Centrifuge Tubes
Common Centrifugation Vocabulary and Formulas
Analytical Ultracentrifuge
Suggested Reading
Important Links

Electrophoresis
Introduction
Principles of GE
Working with the Electrophoresis Apparatus
Denaturing Gradient GE
Temperature Gradient GE
Pulsed-Field GE
Capillary Electrophoresis
Suggested Readings
Important Links

X-Ray Microanalysis
Introduction
Principles
Instrumentation
Applications of X-Ray Microanalysis
Techniques for the Analysis of Secondary, Tertiary, and Quaternary Proteins by X-Ray Crystallography
Suggested Readings
Important Link

Techniques with Radioisotopes
Introduction
Isotopes and Radioactivity
Ionization Effects
Measurement Units
Measurement Techniques
Autoradiography
Counting Statistics
Biological Uses of Radioisotopes
Tracer Dilution Technique
Radioimmunoassay
Miscellaneous Uses of Radioisotopes
Radioisotope Production
Most Widely Used Radioisotopes
Suggested Reading
Important Links

Fermentation
Introduction
History
Principles of Fermentation
Introduction to Microorganisms
Types of Fermentation Metabolism
Fermentation Process
Types of Fermentation
Types of Fermentors
Sterilization in Fermentation
Product Recovery
Suggested Readings
Important Links

Conductivity Meter
Introduction
Principles
Common Definitions
Conductivity Meter
Conductivity Cells
Factors Influencing the Measurement
Measuring Techniques
Reliable Measurements
Maintenance and Storage
Applications
Suggested Readings
Important Links

Glossary

Index

"Designed to serve a multitude section of students and researchers diversified in the field of Life Sciences, Pharmacy, Biotechnology, Microbiology, Biochemistry, and Environmental Sciences, this book addresses the different aspects of instrumentation that hold the key for cutting edge research and innovative application. The authors emphasize techniques encountered both in the practical classes and also in high through put techniques involved in modern era of commercialization and product inventories. It includes illustrated diagrams to explain the principles and theories behind these instruments"--Provided by publisher.

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